EFFECT OF X-IRRADIATION ON SPLEEN NUCLEOTIDE LEVELS

Technical Report · Wed Sep 23 04:00:00 EDT 1959

OSTI ID:4199378

Uyeki, E M

A study was made of the effects of whole-body x irradiation on the adenosine nucleotide levels of the spleens of rats, mice, and guinea pigs. At various intervals after the animals were exposed to 400 and 800 r of x ray, they were sacrificed and measurements taken of the adenosine triphosphate, adenosine diphosphate, and adenylic acid levels. A lethal dose of x ray (800 r) caused a decrease in the ATP levels of rat spleen to 63 percent below normal and a reduction in the total nucleotide content of the spleen. A 50 percert decrease in the adenosine triphosphate levels of the spleens of mice was observed with 400 r of x ray and after 800 r a progressive and continuous decline in this nucleotide occurred during the survival period. The adenosine triphosphate levels of guinea pig spleens decreased more slowly and to a lesser extent than such levels in the spleens of rats and mice. The radiation-induced decreases in adenosine triphosphate content of the spleens of these three species may be explainable on the basis of an increased adenosine triphosphatase activity which was previously observed in this laboratory to be caused by ionizing radiations. The nature of the biologic test system (viz, growing cells) restricts a casual association between the nucleotide titers and phosphatase activity. (auth)

• Research Organization: Chicago. Univ. Air Force Radiation Lab.; Chicago. Univ.
• NSA Number: NSA-14-017688
• OSTI ID: 4199378
• Report Number(s): AF-SAM-60-28
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ADENINE
ADENOSINE
ANIMALS
ATP
BIOLOGY AND MEDICINE
BODY
ENZYMES
GUINEA PIGS
LETHAL DOSE
MICE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC PHOSPHORUS COMPOUNDS
RADIATION DOSES
RADIATION EFFECTS
RATS
SPLEEN
VARIATIONS
X RADIATION