The uptake of calcium-45 and strontium-85 by bone in vitro

Journal Article · Wed Jun 01 04:00:00 EDT 1960 · Archives of Biochemistry and Biophysics

DOI:https://doi.org/10.1016/0003-9861(60)90250-2· OSTI ID:4181928

Samachson, Joseph; Lederer, Hilda

Done shaken at 37 deg C with buffered solutions containing Sr⁸⁵ and Ca⁴⁵ took up more Ca⁴⁵ than Sr⁸⁵, mostly by exchange. The Ca⁴⁵Sr⁸⁵ ratio of 1.2 to 1.4, in the absence of carrier or in the presence of stable Ca, rose to 2.0 or higher as the stable Sr concentration was increased. Human and canine bone powder, pieces of bone, ashed bone, bone treated wlth ethylenediamine, and apatite all behaved similarly. The additlon of inorganic phosphate and changes in pH dld not affect the ratio. Ca⁸⁵/Sr⁸⁵ uptake ratios from beef serum and seram ultrafiltrate were close to 1 in the absence of Sr carrier, apparently because stronger binding of Ca⁴⁵ by serum proteins and chelating agents compensated for preferential Ca⁴⁵ uptake by bone. Implications of the results are discussed wlth regard to the use of Sr as an adjuvant in osteoporosis, Sr⁸⁵ and stable Sr as tracers for Ca, and the uptake of Sr⁹⁰ by bone.

• Research Organization: Montefiore Hospital, New York
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-016507
• OSTI ID: 4181928
• Journal Information: Archives of Biochemistry and Biophysics, Journal Name: Archives of Biochemistry and Biophysics Journal Issue: 2 Vol. 88; ISSN 0003-9861
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACIDITY
APATITES
BLOOD SERUM
BONES
CALCIUM 45
CALCIUM PHOSPHATES
DISEASES
DOGS
EDTA
MAN
METABOLISM
MINERALS
PHOSPHATES
PROTEINS
QUANTITY RATIO
SOLUTIONS
STRONTIUM 85
STRONTIUM 90
TEMPERATURE
TESTING
VARIATIONS