EFFECTS OF IMMUNIZED RAT BONE MARROW ON LETHALLY IRRADIATED MICE

Technical Report · Fri Apr 29 04:00:00 EDT 1960

OSTI ID:4169543

Chin, P C; Silverman, M S

The time and the incidence of deaths between lethally irradiated mice treated either with non-immune rate bone marrow or treated with marrow from nats pre-immunized against the recipient mice were compared for the purpose of determining the role of the donor cells in the pathogenesis of secondary deaths. On the basis of a graft vs. host reaction, enhancement of the time and incidence of secondary deaths would be expected, due to the heightened immunological reactivity of the pre-immunized marrow against the host. In contract, a host vs. graft reaction or the unsuccessful colonization of immunologically active donor cells will result in a lack of difference between recipients of either non-immune or pre-immunized marrow. Statistical analysis of the results from the groups of animals in each of three different bone marrow experiments showed no significant difference in either the time or the incidence of deaths. Furthermore, this lack of significant difference was observed even when moderate amounts of either non- immune or pre-immunized rat spleen were administered to recipients of non-immune rate bone marrow. These results suggest that following the injection of rat bone marrow proliferation of immunologically active cells either did not take place or occurred only to a very limited extent. In either instance, a graft vs. host reaction would be unlikely. (auth)

• Research Organization: Naval Radiological Defense Lab., San Francisco
• NSA Number: NSA-14-014664
• OSTI ID: 4169543
• Report Number(s): USNRDL-TR-417
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
BONE MARROW
IMMUNITY
IRRADIATION
LETHAL DOSE
MICE
RATS
SPLEEN
TRANSPLANTS