OBSERVATIONS OF THE PASSIVE TRANSFER OF TRANSPLANTATION IMMUNITY AND DELAYED HYPERSENSITIVITY WITH LYMPHOID CELLS IN MILLIPORE CHAMBERS

Journal Article · Tue Oct 01 00:00:00 EDT 1963 · Transplantation See Ishoku

DOI:https://doi.org/10.1097/00007890-196301040-00007· OSTI ID:4164702

Najarian, J S; Feldman, J D

Experiments were done to determine whether antigen or a transfer factor escaped from millipore chambers containing viable cells with subsequent immunization of the recipient mice. Homologous nonsensitized and isologous sensitized lymphoid cells enclosed in millipore chambers were transferred to recipient mice. After appropriate time intervals, the hosts were tested by skin homografts. There was no evidence that recipients were immunized by an escape from the chambers of homologous cells, of antigen carried over with sensitized cells, or of a transfer factor. In mice given 650 r total-body irradiation, a dose sufficient to prolong the MST of first-set grafts by 6 days, accelerated rejection of test skin hornografts was achieved by lymphoid cells sensitized to homologous tissues and enclosed in millipore chambers. That leakage of cells might occur, but in numbers too small to effect immunization of the host, was indicated by the deaths of 5 of 53 recipients of virulent leukemia cells placed in chambers. In guinea pigs, tuberculin and contact chemical sensitivity could not be transferred by cells sensitized to tubercle bacilli or dinitrofluorobenzene and contained in cell-impenetrable millipore chambers. These data corroborated the conclusion that transplantation immunity on the one hand and tuberculin and contact sensitivity on the other hand are mediated by different immunologic mechanisms. (auth)

• Research Organization: Scripps Clinic and Research Foundation, La Jolla, Calif.
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-000115
• OSTI ID: 4164702
• Journal Information: Transplantation See Ishoku, Vol. Vol: 1; Other Information: Orig. Receipt Date: 31-DEC-64
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
ANIMAL CELLS
ANTIGENS
BACTERIA
BENZENE
BINDING ENERGY
BODY
CHEMICALS
CRYSTALS
ELECTRONS
ETHYL RADICALS
FREE RADICALS
GAMMA SOURCES
GUINEA PIGS
IMMUNITY
IRRADIATION
LATTICES
LEUKEMIA
LYMPHOCYTES
MICE
MYCOBACTERIUM TUBERCULOSIS
ORGANIC ACIDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION DOSES
RADIATION EFFECTS
RESONANCE
SKIN
SPIN
TRANSPLANTS
TUBERCULIN