ANTI-BOVINE SERUM ALBUMIN FORMATION BY TRANSFERRED HYPERIMMUNE MOUSE SPLEEN CELLS
Antibody formation by transferred hyperimmune spleen cells was studied using bovine serum albumin as antigen and inbred mice as donors and recipients. It was shown that isologous adult and neonatal hosts support antibody formation by transferred cells. The amount of antibody formed in neonatal hosts was less than that in adult recipients. Antibody was not formed in homologous adult or newborn recipients, and this is most likely because of rejection of the transferred cells prior to their formation of antibody. The host-vs-graft reaction could be suppressed by prior x irradiation of recipients with consequent antibody formation by transferred homologous cells. Further, if isologous recipients were irradiated prior to cell transfer, the antibody response was found to be greater than that in nonirradiated isologous hosts. Transferred cells appeared to survive in the isologous recipients for a prolonged period of time with continuing formation of antibody. It may be that after depletion of lymphoid tissue by radiation, there is a nonspecific stimulus for proliferation of lymphoid cells which affects both the remaining host lymphoid cells and the transferred cells. An additional possibility is that in conjunction with decreased competition from the host's own cells, the antigen stimulated anti-BSA producing cells, which are capable of more rapid division than unstimulated cells, are consequently able to occupy a greater proportion of the total lymphoid space than the unstimulated lymphoid cells. The lesser antibody response by transferred cells in isologous hosts with delay of restimulation suggests that the number of transferred cells capable of responding to antigen decreases with time after transfer. (BBB)
- Research Organization:
- Scripps Clinic and Research Foundation, La Jolla, Calif.
- NSA Number:
- NSA-18-015855
- OSTI ID:
- 4077971
- Journal Information:
- Journal of Immunology (U.S.), Journal Name: Journal of Immunology (U.S.) Vol. Vol: 91; ISSN JOIMA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
TRANSFER OF RABBIT LYMPH NODE CELLS TO NEONATAL RECIPIENT RABBITS
ANTIBODY PRODUCTION BY IMMUNOLOGICALLY COMPETENT CELLS TRANSFERRED TO A HETEROLOGOUS HOST