Induction of chromosomal aberrations in human lymphocytes by x rays and fission neutrons: dependence on cell cycle stage

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · Radiat. Res., v. 63, no. 3, pp. 403-421

DOI:https://doi.org/10.2307/3574093· OSTI ID:4157479

Carrano, A V

Human lymphocytes were irradiated in culture with several doses of 250 kVp x rays or fission neutrons (0.85 MeV average energy) either in the unstimulated state (G$sub 0$), at 17 hr after phytohemagglutinin stimulation (G$sub 1$), or at approximately 48 hr after stimulation. In the last case, the cells were labeled with tritiated thymidine prior to irradiation to distinguish S and G$sub 2$ cells. All cultures were harvested at two successive intervals between 48 and 58 hr. The frequencies of neutron-induced chromatid deletions in G$sub 2$ cells differed significantly among harvest times and definite trends were observed for other aberrations. Dose-response curves for chromosome type aberrations followed the classical aberration production model; chromatid aberrations exhibited more complex kinetics. The differential cell cycle response to aberration induction varied with radiation quality, dose, and aberration type. The relative biological effectiveness of fission neutrons for aberration production also varied with aberration type, cell cycle stage, and neutron dose but remained principally between 2 and 6. (auth)

• Research Organization: Univ. of California, Livermore
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-007484
• OSTI ID: 4157479
• Journal Information: Radiat. Res., v. 63, no. 3, pp. 403-421, Journal Name: Radiat. Res., v. 63, no. 3, pp. 403-421; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

*CELL CYCLE-- RADIOSENSITIVITY EFFECTS
*CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION
*FISSION NEUTRONS-- RBE
*LYMPHOCYTES-- BIOLOGICAL RADIATION EFFECTS
560151* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Man
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
IRRADIATION
KEV RANGE 100- 1000
MAN
N48510* --Life Sciences--Radiation Effects on Animals--Man
RADIATION QUALITY
TIME DEPENDENCE
X RADIATION