Nucleic Acid Metabolism in HeLa S3 Cells after X-Ray-Induced Mitotic Delay
The nucleic acid metabolism of HeLa S3 cells after x-radiation-induced mitotic delay was investigated. A depression in the percentage of cells in DNA synthesis accompanies resumption of mitosis, but is followed by a return to near- normal values in the evolving glant-cell populatlon. As the number of cells in the population decreases, owing to abortive divisions, the percentage of cells in synthesis slowly decreases. One of the effects of irraddation appears to be a mixing of the cells in the S and G/sub 2/phases of the population at the time of irradiation, so that cells from both compartments are in the population of cells that begin dividing after mitotic delay. The metabolism of RNA is little affected by doses of nediation up to 1500 r, and for times up to at least 14 days after the irradiation. After a short incubatlon with tritiated cytidine, the norrmal sequence of chromatin, nucleolar, and cytoplasmic labeling occurs in qulalitatively the same manner in almost all irradiated cells, even the huge glants foumd 7 to 14 days after irradiation, as it does in unirradiated controls.
- Research Organization:
- Battelle Memorial Inst., Columbus, Ohio
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-002495
- OSTI ID:
- 4151774
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 5 Vol. 13; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Effect of Irradiation and Theory of Role of Mitotic Delay on the Time Course of Labeling of HeLa S3 Cells with Tritiated Thymidine
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