ACTION OF RADIATION ON MAMMALIAN CELLS, IV. REVERSIBLE MITOTIC LAG IN THE S3 HELA CELL PRODUCED BY LOW DOSES OF X-RAYS

Journal Article · Tue Aug 01 00:00:00 EDT 1961 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.47.8.1181· OSTI ID:4843742

Yamada, M. -a.; Puck, T. T.

The life cycle of hyperploid S3 HeLa cells, irradiated with sublethal doses of x rays, was studied. A reversible mitotic lag was readily demonstrated at doses of 34 to 135 rads, prcduced by a block localized in the G2, pre-mitotic period. Visible chromosomal aberrations were produced in a majority of the cell population at these doses. It was proposed that this reversible mitotic lag, like irreversible reproductive death, is due to chromosomal damage and that the reversible lag may reflect interference with chromosomal condensation just prior to and perhaps in the early parts of the mitosis. (auth)

• Research Organization: Univ. of Colorado Medical Center, Denver
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-027468
• OSTI ID: 4843742
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Vol. 47, Issue 8; Other Information: Orig. Receipt Date: 31-DEC-61; ISSN 0027-8424
• Publisher: National Academy of Sciences, Washington, DC (United States)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
ANIMAL CELLS
CHROMOSOMES
MITOSIS
X RADIATION