Effect of Irradiation and Theory of Role of Mitotic Delay on the Time Course of Labeling of HeLa S3 Cells with Tritiated Thymidine

Journal Article · Sat Aug 01 04:00:00 EDT 1959 · Radiation Research

DOI:https://doi.org/10.2307/3570659· OSTI ID:4255523

Painter, Robert B.; Robertson, James S.

The percentage of HeLa S3 cells in DNA synthesis at various times after 500 r of x irradiation has been determined by means of autoradiography with H³-thymidine. The percentage rises during the period of mitotic delay so that at 4 to 8 hours after irradiation almost twice the proportion of cells are synthesizing DNA as are doing so in controls. In an attempt to explain these results on the basis of the build-up of cells in DNA synthesis occurring as a result of mitotic delay, the kinetics of the effects of irradiation on the numbers of cells in the various stages of the mitotic cycle have been determined. These kinetics are discussed with respect to their importance in the interpretation of data on the effects of irradiation of DNA synthesis, but they fail to account completely for the results reported here. Alternatively the interpretation is that many cells in DNA synthesis at time of x irradiation remain in this stage for an abnormally long time and there is no effect on the rate at which cells come into DNA synthesis.

• Research Organization: Brookhaven National Lab., Upton, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-13-018842
• OSTI ID: 4255523
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 11; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
CANCER
CONTROL
CYTOLOGY
DNA
MITOSIS
NUCLEIC ACIDS
RADIATION EFFECTS
RADIOAUTOGRAPHY
THYMIDINE
TRITIUM
X RADIATION