THE PREPARATION OF $sup 131$I-LABELED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY

Journal Article · Tue Oct 01 04:00:00 EDT 1963 · Biochemical Journal (England)

DOI:https://doi.org/10.1042/bj0890114· OSTI ID:4150450

Greenwood, F C; Hunter, W M; Glover, J S

A simple and rapid method is presented for the preparation of I/sup 131/- labeled human growth hormone of high specific radioactivity (240-300 mu C/ mu g). Low amounts of carrierfree I/sup 131/ iodide (2 mC) are allowed to react, without prior treatment, with small quantities of protein (5 mu g) in a highyield reaction (approx. 70% transfer of I/sup 131/ to protein). The degree of chemical substitution is minimized (0.5- 1.0 atom of iodine/molecule of protein) by the use of carrier-free I/sup 131/ iodide. The I/sup 131/-labeled hormone (up to 300 mu C/ mu g) contains no detectable degradation products and is immunologically identical with the unlabeled hormone. The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated. (auth)

• Research Organization: Imperial Coll. of Science and Tech., London
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-001328
• OSTI ID: 4150450
• Journal Information: Biochemical Journal (England), Journal Name: Biochemical Journal (England) Vol. Vol: 89; ISSN BIJOA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ATOMS
BIOLOGY AND MEDICINE
CHEMICAL REACTIONS
HORMONES
IMMUNITY
IODIDES
IODINE 127
IODINE 131
LABELLED COMPOUNDS
MAN
MOLECULES
PREPARATION
PROTEINS
QUANTITY RATIO
RADIOACTIVITY
STH
VARIATIONS