SUPPRESSION BY CYSTEAMINE OF RADIOSENSITIZATION IN 5-BROMODEOXYURIDINE SUBSTITUTED PHAGE T1

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Biochemical and Biophysical Research Communications (U.S.)

DOI:https://doi.org/10.1016/0006-291X(63)90129-3· OSTI ID:4150406

Hotz, G

Recently it was shown that the formation of a free radical by ionizing radiation in thymine requires 1200 ev but in 5-bromouracil only 150 ev. On the strength that this finding may be of importance for designing a model for the sensitizing action of halogen-substituted DNA-bases, an investigation was made of the effect of a sulfhydryl compound on preventing or repairing particularly those additional radiolesions observed in BUDR-labeled phage. In order to replace thymidine by 5-bromodeoxyuridine, T1 wildtype phage was grown according to the method of Stohl et al. Irradiation was accomplished via a Co/sup 60/ gammasource and a 6-watt Hanau low-pressure uv-lamp. Results indicate that: the sensitizing effect is observed in phage irradiated with uv as well as gamma ; BUDR sensitizes T1 by equal factors (about 2) independently of the conditions for ionizing radiation; cysteamine, if present during irradiation, removes completely the radiosensitization in wet BUDR-phage, but not in dry systems. It was concluded that radiosensitization due to incorporation of halogen-substituted base analoinies into phage-DNA has the same origin for uv and ionizing radiation. (H.M.G.)

• Research Organization: Kernforschungszentrum, Karlsruhe, Ger.
• NSA Number: NSA-18-001449
• OSTI ID: 4150406
• Report Number(s): KFK-161; 0006-291X
• Journal Information: Biochemical and Biophysical Research Communications (U.S.), Journal Name: Biochemical and Biophysical Research Communications (U.S.) Vol. Vol: 11; ISSN BBRCA
• Country of Publication: Germany
• Language: English

Related Subjects

AMINES
AMINO ACIDS
BACTERIOPHAGES
BIOLOGY AND MEDICINE
COBALT 60
CYSTEAMINE
DNA
ENERGY
FREE RADICALS
GAMMA RADIATION
HALOGENS
HETEROCYCLICS
HUMIDITY
HYDROXIDES
KETONES
MEA
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRESSURE
PYRIMIDINES
RADIATION EFFECTS
RADIATION SOURCES
RADIOSENSITIVITY
SULFUR HYDRIDES
THYMINE
ULTRAVIOLET RADIATION
URACILS
VARIATIONS
X RADIATION