Influence of cysteamine on intramolecular energy transfer in 5-bromouracil- substituted phage-DNA

Journal Article · Thu Nov 01 04:00:00 EST 1973 · Int. J. Radiat. Biol., v. 24, no. 5, pp. 517-524

DOI:https://doi.org/10.1080/09553007314551401· OSTI ID:4350248

Moenkehaus, F

With the aid of an analytical ultracentrifuge, single-and doublestrand breakage rates of 5-bromouracil (BrU)-substituted DNA of a defective B. subtilis phage (PBSH) were determined after irradiation with uv (302 to 3l3 mn) in the presence and absence of cysteamine. The presence of cysteamine reduced the appearance of single-strard breaks (10%) in the BrU-containing strands of hybrid and bifilarly-substituted DNA. The primary breaks lead to secondary breaks in the complementary thymine-containing strand of hybrid DNA or in the complementary BrU-containing strand of bifilarly-substituted DNA (intramolecular enengy transfer). Cysteamine strongly reduced (< 1%) these secondary breaks, and it is suggested that this indicates that radical reactions are involved in the mechanism of intramolecular energy transfer. (auth)

• Research Organization: Univ., Muenster, Ger.
• NSA Number: NSA-29-015833
• OSTI ID: 4350248
• Journal Information: Int. J. Radiat. Biol., v. 24, no. 5, pp. 517-524, Journal Name: Int. J. Radiat. Biol., v. 24, no. 5, pp. 517-524; ISSN IJRBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*BACTERIOPHAGES-- BIOLOGICAL RADIATION EFFECTS
*DNA-- STRAND BREAKS
*MEA-- RADIOSENSITIVITY EFFECTS
*STRAND BREAKS-- RADIOINDUCTION
BACILLUS SUBTILIS
BROMOURACILS
ENERGY TRANSFER
N48120* --Life Sciences--Radiation Effects on Biochemicals-- In Microorganisms
N48310 --Life Sciences--Radiation Effects on Microorganisms--Basic Studies
PHOTON BEAMS
RADICALS
THYMINE
ULTRACENTRIFUGATION
ULTRAVIOLET RADIATION