STUDIES ON DEOXYRIBONUCLEIC ACID AFTER EXPOSURE TO TRITIUM GAS

Journal Article · Thu Jan 01 04:00:00 EST 1959 · Journal of Molecular Biology (England)

DOI:https://doi.org/10.1016/S0022-2836(59)80026-7· OSTI ID:4143240

Borenfreund, E; Rosenkranz, H S; Bendich, A

Deoxyribonucleic acid (DNA) from human leukocytes was exposed to 0.39 atm. of tritium (2.4 curies) for 14 days at room temperature, and approximately 0.1% replacement of hydrogen by tritium was effected. The molar ratios of tritium in the bases was adenine 1.0, thymine 9.8, guanine 1.4, and crosine 2.4. The treatment caused a drop in average moleculsr weight from 5.2 to 1.3 x 10/sup 6/ as estimated from sedimentation data and there was a concomitant alteration in the anion exchange chromatographic behavior. Evidence was also obtained for some aggregation in addition to the degradation. These macromolecular changes did not appear to be accompanied by significant denaturation. (auth)

• Research Organization: Cornell Univ. Medical Coll., New York
• Sponsoring Organization: USDOE
• NSA Number: NSA-14-013554
• OSTI ID: 4143240
• Journal Information: Journal of Molecular Biology (England), Journal Name: Journal of Molecular Biology (England) Vol. Vol: 1; ISSN JMOBA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ADENINE
ANIONS
BIOLOGY AND MEDICINE
CHROMATOGRAPHY
CYTOSINE
DECOMPOSITION
DNA
GASES
GUANINE
HETEROCYCLICS
HYDROGEN
ION EXCHANGE
LEUCOCYTES
MAN
MOLECULES
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PRECIPITATION
PYRIMIDINES
TEMPERATURE
THYMINE
TRITIUM
VARIATIONS
VITAMIN B-4
VITAMINS
WEIGHT