EFFECT OF IONIZING RADIATION ON DEOXYRIBONUCLEIC ACID. III. THE STRUCTURE OF HIGHLY POLYMERIZED DEOXYRIBONUCLEIC ACID FROM IRRADIATED RAT SPLEEN
Journal Article
·
· Folia Biologica (Prague) (Czechoslovakia)
OSTI ID:4062544
The structure of desoxyribonucleic acid (DNA) isolated by the Schmidt- Thannhauser method from the spleen of rats irradiated with a dose of 400 r was investigated. Five days after irradiation the guanine/cytosine quotient and the adenine/thymine quotient were approximately two and after 18 days were still higher than one. The two quotients did not again equal the values in nonirradiated animals until 30 days after irradiation. In DNA isolated in a highly polymerized form from the same source s, the guanine/cytosine quotient and the adenine/thymine quotient were also about equal to one five days after irradiation. The increase ~n the purine/pyrimidine quotient in DNA isolated by the SchmictThannhauser method is attributed to the presence of products of the enzymatic hydrolysis of DNA. (auth)
- Research Organization:
- Inst. of Biophysics, Academy of Sciences, Brno
- NSA Number:
- NSA-15-014130
- OSTI ID:
- 4062544
- Journal Information:
- Folia Biologica (Prague) (Czechoslovakia), Journal Name: Folia Biologica (Prague) (Czechoslovakia) Vol. Vol: 7; ISSN FOBLA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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