Characterization of rat liver ribonucleic acids: Preliminary nucleotide analyses of subcellular fractions of resting and regenerating rat liver
This paper deals with the preparation of rat liver homogenates from both normal and regenerating liver, their fractionation by the method of Schneider, and the nucleotide analyses of the ribonucleic acid fractions, recovered by the method of Schmidt and Thannhauser. The ribonucleic acid from the total cell homogenate was also isolated and analyzed. Guanine to adenine ratios from 2.5 to 4.5 and pyrimidine to purine ratios of 1.5 to 2.5 were found. The uridylic to cytidylic acid ratios were found to be unreliable. The predominant differences observed between resting and regenerating liver were a lower pyrimidine to purine ratio and the presence of a greater percentage of the phosphorus in the more difficulty sedimentable fractions in the regenerating liver. Suggestions for further work are also made. 30 refs., 5 tabs.
- Research Organization:
- Chicago Univ., IL (United States)
- OSTI ID:
- 182182
- Report Number(s):
- COO--22; ON: TI95013638
- Country of Publication:
- United States
- Language:
- English
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