SPONTANEOUS CHROMOSOMAL INJURIES OF EMBRYONIC HUMAN FIBROBLASTS IN VIVO AND IN VITRO (in Russian)
Five human embryos 1.5 to 2 months old were used in order to determine the spontaneous mutation rate in man by counting the chromosomal aberrations. A primary cell culture of the embryonic fibroblasts was also used to determine the gamma radiation dose required to double the spontaneous mutation rate in man. The chromosomal aberrations were counted during anaphase and telophase. The spontaneous mutation rate varied from almost zero for embryo No. 4 to 0.56% for embryo No. 3 with an average of 0.28% for the five embryos. The spontaneous mutation rate in vitro was higher by a factor of 6 than the mutation rate in vivo, and varied from 1.3 to 2.0% with an average of 1.6%. The average dose doubling the natural mutation rate in embryonic human fibroblasts varied from 1.3 to 2.1 r in vivo, and from 7.2 to 12.3 F in vitro. (TTT)
- Research Organization:
- Inst. of Biological Physics, Academy of Sciences, USSR
- NSA Number:
- NSA-18-008149
- OSTI ID:
- 4132614
- Journal Information:
- Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals, Journal Name: Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals Vol. Vol: 153; ISSN DANKA
- Country of Publication:
- Country unknown/Code not available
- Language:
- Russian
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