Effect of extreme hypoxia on the repair of DNA single-strand breaks in mammalian cells

Journal Article · Sat Nov 01 04:00:00 EST 1975 · Radiat. Res., v. 64, no. 2, pp. 256-269

DOI:https://doi.org/10.2307/3574263· OSTI ID:4125475

Koch, C J; Painter, R B

About 20 percent of the single-strand breaks produced by x rays in V79 Chinese hamster cells under extremely hypoxic conditions remain unrepaired after 1 hr at 37$sup 0$C. The corresponding figure is only 10 percent for cells irradiated under moderately hypoxic (0.2 $mu$M) or aerobic conditions (200 $mu$M). The initial number of breaks produced per unit dose is identical for extremely hypoxic and moderately hypoxic cells and is about four times greater for aerobic cells. Because split-dose recovery proceeds under aerobic and moderately hypoxic conditions and is inhibited only under extremely hypoxic conditions, the split- dose recovery phenomenon may be related to the unrepaired component of single- strand breaks. (auth)

• Research Organization: Univ. of California, San Francisco
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-017312
• OSTI ID: 4125475
• Journal Information: Radiat. Res., v. 64, no. 2, pp. 256-269, Journal Name: Radiat. Res., v. 64, no. 2, pp. 256-269; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*ANOXIA-- RADIOSENSITIVITY EFFECTS
*DNA-- STRAND BREAKS
*STRAND BREAKS-- BIOLOGICAL REPAIR
560121* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Cells--External Source
CELL CULTURES
HAMSTERS
IRRADIATION
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
X RADIATION