Repair of DNA double-strand breaks in irradiated yeast cells under nongrowth conditions
Journal Article
·
· Radiat. Res.; (United States)
We show that in diploid yeast radiation-induced DNA double-strand breaks can be repaired under nongrowth conditions at 30/sup 0/C and that liquid holding recovery of colony-forming ability is accompanied by a decrease in the number of double-strand breaks per cell. The kinetics of double-strand break repair under nongrowth conditions depend on the dose applied. For the highest dose used (2400 Gy) repair is completed within about 48 h. We have verified that double-strand breaks which remain unrepaired after 48 h are irreparable under nongrowth conditions. The linear relationship between the initial number of double-strand breaks and dose is converted into a quadratic function of irreparable double-strand breaks with and dose during liquid holding treatment of cells.
- Research Organization:
- Gesellschaft fuer Strahlen-und Umweltforschung mbH, Munich, Germany
- OSTI ID:
- 5129612
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 82:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550700 -- Microbiology
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COLONY FORMATION
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
FRACTIONATED IRRADIATION
FUNGI
GROWTH
INHIBITION
IONIZING RADIATIONS
IRRADIATION
LEPTONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
YEASTS
550700 -- Microbiology
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COLONY FORMATION
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
FRACTIONATED IRRADIATION
FUNGI
GROWTH
INHIBITION
IONIZING RADIATIONS
IRRADIATION
LEPTONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
YEASTS