Tritiated-thymidine induced changes in cell population kinetics in root meristems of Pisum sativum

Journal Article · Mon Dec 01 04:00:00 EST 1975 · Radiat. Res., v. 64, no. 3, pp. 475-484

DOI:https://doi.org/10.2307/3574237· OSTI ID:4102072

Stetka, D G; Webster, P L

The effects of $sup 3$H-thymidine on cell progression through the mitotic cycle and on cell population kinetics have been investigated. During continuous treatment there is a gradual inhibition of entry of cells from G2 into mitosis, while other stages of the cell cycle appear to be less radiosensitive. Hence there is an accumulation of cells in G2 while the proportions of cells in G1, S, and M decrease. Cells of the quiescent center are induced to synthesize DNA after inhibitions of mitosis in the surrounding meristem. They do not serve to repopulate the meristem, however, since they then incorporate $sup 3$H- thymidine and are themselves arrested. It is suggested that the other slowly cycling cells of the meristem, in addition to those of the quiescent center, may also be induced to cycle more rapidly after delay in the fast-cycling population. These cells will also be arrested after a brief compensatory proliferative reaction. Thus the meristem is unable to recover from radiation damage during continuous treatment with $sup 3$H-thymidine. (auth)

• Research Organization: Univ. of Massachusetts, Amherst
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-015104
• OSTI ID: 4102072
• Journal Information: Radiat. Res., v. 64, no. 3, pp. 475-484, Journal Name: Radiat. Res., v. 64, no. 3, pp. 475-484; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

*CELL CYCLE-- BIOLOGICAL RADIATION EFFECTS
560163* --Biomedical Sciences
Applied Studies--Radiation Effects--Radionuclide Effects--Internal Source--Plants
INTERNAL IRRADIATION
MERISTEMS
N48630* --Life Sciences--Radionuclide Effects (Internal Source)--Plants
PISUM
PLANT CELLS
ROOTS
THYMIDINE
TRITIUM
TRITIUM COMPOUNDS