Cell population kinetics of excised roots of Pisum sativum

Journal Article · Fri Oct 01 00:00:00 EDT 1965 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.27.1.179· OSTI ID:1629145

Van't Hof, Jack ^[1]

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1. Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.; DOE/OSTI

The cell population kinetics of excised, cultured pea roots was studied with the use of tritiated thymidine and colchicine to determine (1) the influence of excision, (2) the influence of sucrose concentration, (3) the average mitotic cycle duration, and (4) the duration of mitosis and the G1, S, and G2 periods of interphase.1 The results indicate that the process of excision causes a drop in the frequency of mitotic figures when performed either at the beginning of the culture period or after 100 hours in culture. This initial decrease in frequency of cell division is independent of sucrose concentration, but the subsequent rise in frequency of division, after 12 hours in culture, is dependent upon sucrose concentration. Two per cent sucrose maintains the shortest mitotic cycle duration. The use of colchicine indicated an average cycle duration of 20 hours, whereas the use of tritiated thymidine produced an average cycle duration of 17 hours.

• Research Organization: Brookhaven National Laboratory (BNL), Upton, NY (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
• Grant/Contract Number: AC02-98CH10886
• OSTI ID: 1629145
• Journal Information: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 27; ISSN 0021-9525
• Publisher: Rockefeller University Press
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES