Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

The mechanism of 5-aminouracil-induced synchrony of cell division in Vicia faba root meristems

Journal Article · · Journal of Cell Biology
DOI:https://doi.org/10.1083/jcb.24.3.401· OSTI ID:1629176
 [1];  [2]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.; DOE/OSTI
  2. Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.
+ Show Author Affiliations

Cessation of mitosis was brought about in Vicia faba roots incubated for 24 hours in the thymine analogue, 5-aminouracil. Recovery of mitotic activity began 8 hours after removal from 5-aminouracil and reached a peak at 15 hours. If colchicine was added 4 hours before the peak of mitoses, up to 80 per cent of all cells accumulated in mitotic division stages. By use of single and double labeling techniques, it was shown that synchrony of cell divisions resulted from depression in the rate of DNA synthesis by 5-aminouracil, which brought about an accumulation of cells in the S phase of the cell cycle. Treatment with 5-aminouracil may have also caused a delay in the rate of exit of cells from the G2 period. It appeared to have no effect on the duration of the G1 period. When roots were removed from 5-aminouracil, DNA synthesis resumed in all cells in the S phase. Although thymidine antagonized the effects of 5-aminouracil, an exogenous supply of it was not necessary for the resumption of DNA synthesis, as shown by incorporation studies with tritiated deoxycytidine.

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
SC0012704
OSTI ID:
1629176
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 3 Vol. 24; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

References (1)

The Relation Between dna Synthesis and Chromosome Structure as Resolved by X-Ray Damage journal September 1963

Cited By (3)

Hydroxyurea-induced mitotic synchronization in Allium sativum root meristems journal November 1980
High sensitivity of DNA replication to 5-aminouracil in the middle of the S period journal February 1984
Synthesis, reactivity, and biological activity of 5-aminouracil and its derivatives journal April 2015

Similar Records

INCORPORATION OF $sup 3$H-ARGININE IN CHROMOSOMES OF VICIA FABA
Technical Report · Mon Dec 31 23:00:00 EST 1962 · OSTI ID:4026233
Mechanisms of reorganization and cell repopulation in meristems in roots of Vicia faba following irradiation and colchicine
Journal Article · Sat Dec 31 23:00:00 EST 1960 · Chromosoma · OSTI ID:4802876
THE RECOVERY OF VICIA FABA MERISTEM CELLS FROM X-RADIATION
Journal Article · Tue Oct 01 00:00:00 EDT 1963 · Radiation Res. · OSTI ID:4703963

Related Subjects

59 BASIC BIOLOGICAL SCIENCES