Inactivation of carotenoid-producing and albino strains of Neurospora crassa by visible light, blacklight, and ultraviolet radiation
Journal Article
·
· J. Bacteriol., v. 125, no. 2, pp. 616-625
OSTI ID:4093578
Suspensions of Neurospora crassa conidia were inactivated by blacklight (BL) radiation (300 to 425 nm) in the absence of exogenous photosensitizing compounds. Carotenoid-containing wild-type conidia were less sensitive to BL radiation than albino conidia, showing a dose enhancement factor (DEF) of 1.2 for dose levels resulting in less than 10 percent survival. The same strains were about equally sensitive to shortwave ultraviolet (uv) inactivation. The kinetics of BL inactivation are similar to those of photodynamic inactivation by visible light in the presence of a photosensitizing dye (methylene blue). Only limited inactivation by visible light in the absence of exogenous photosensitizers was observed. BL and UV inactivations are probably caused by different mechanisms since wild-type conidia are only slightly more resistant to BL radiation (DEF = 1.2 at 1.0 percent survival) than are conidia from a uv-sensitive strain (upr-1, uvs-3). The BL-induced lethal lesions are probably not cyclobutyl pyrimidine dimers since BL-inactivated Haemophilus influenzae transforming deoxyribonucleic acid is not photoreactivated by N. crassa wild-type enzyme extracts, whereas uv- inactivated transforming deoxyribonucleic acid is photoreactivable with this treatment. (auth)
- Research Organization:
- Univ. of Illinois, Urbana
- NSA Number:
- NSA-33-020489
- OSTI ID:
- 4093578
- Journal Information:
- J. Bacteriol., v. 125, no. 2, pp. 616-625, Journal Name: J. Bacteriol., v. 125, no. 2, pp. 616-625; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ultraviolet-inactivation of conidia from heterokaryons of Neurospora crassa containing uv-sensitive mutations
Isolation of MMS- and histidine-sensitive mutants in Neurospora crassa
ABSENCE OF INTERACTION BETWEEN NITROGEN MUSTARD AND VISIBLE RADIATION IN MUTATION INDUCTION IN NEUROSPORA CRASSA
Conference
·
Sun Dec 31 23:00:00 EST 1972
·
OSTI ID:4312433
Isolation of MMS- and histidine-sensitive mutants in Neurospora crassa
Journal Article
·
Sat Jan 31 23:00:00 EST 1981
· Genetics; (United States)
·
OSTI ID:6123100
ABSENCE OF INTERACTION BETWEEN NITROGEN MUSTARD AND VISIBLE RADIATION IN MUTATION INDUCTION IN NEUROSPORA CRASSA
Journal Article
·
Wed Oct 31 23:00:00 EST 1962
· Radiation Botany
·
OSTI ID:4720861
Related Subjects
*CONIDIA-- BIOLOGICAL RADIATION EFFECTS
*NEUROSPORA-- PHOTOSENSITIVITY
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
DOSE-RESPONSE RELATIONSHIPS
METHYLENE BLUE
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION
VISIBLE RADIATION
*NEUROSPORA-- PHOTOSENSITIVITY
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
DOSE-RESPONSE RELATIONSHIPS
METHYLENE BLUE
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION
VISIBLE RADIATION