Isolation of MMS- and histidine-sensitive mutants in Neurospora crassa

Journal Article · Sun Feb 01 04:00:00 EST 1981 · Genetics; (United States)

OSTI ID:6123100

Delange, A M; Mishra, N C

A simple method of replica plating has been used to isolate mutants of Neurospora crassa that have increased sensitivity to methyl methanesulfonate (MMS) and/or to histidine. Twelve mutants with increased sensitivity to MMS and one mutant with increased sensitivity to histidine showed Mendelian segregation of the mutant phenotypes. Three mutants were mapped to loci not previously associated with MMS sensitivity. Two others were allelic to the uv- and MMS-sensitive mutant, mei-3. Survival curves indicate that conidia (mutant or wild-type) survive on much higher concentrations of MMS at 25/sup 0/ than at 37/sup 0/. In contrast, mycelial growth is more resistant to MMS at 37/sup 0/. The possibility of qualitatively different repair processes at these two temperatures is discussed.

• Research Organization: Univ. of South Carolina, Columbia
• OSTI ID: 6123100
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 97:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACIDS
DNA
ELECTROMAGNETIC RADIATION
ESTERS
FUNGI
GENES
GENETIC MAPPING
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
MAPPING
METHYL METHANESULFONATE
MUTAGENS
MUTANTS
NEUROSPORA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTYPE
PLANTS
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION