THE BEHAVIOR OF RADIOIODINE ACTIVITY IN THE BLOOD AND IN ANIMAL ORGANS UNDER THE INFLUENCE OF L-CARNITINE

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Acta Biologica et Medica Germanica (East Germany)

OSTI ID:4075785

Rotzsch, W; Willgerodt, H

Previous experiments showed a partially antagonistic influence of carnitine on thyroid hormones. Carnitine lowers the elevated basal metabolic rate in hypenthyroidism, brings the vitamin B concentrations (increased by thyroxin in the skeletal muscles) into physiologic range, and influences protein metabolism which is altered by thyroid hormones. To elucidate this effect, the body distribution of I/sup 131/ was investigated after irtroducing carnitine. The hormonal protein-bound iodine in serum, electrophoretically determined, indicated significant quantitative differences in mice and rats after carnitine treatment, pointing to a basic difference in iodine metabolism. Albino mice body wt 20 g) and Fisher strain rats were injected intraperitoneally with L-carnitine in the form of free betaine (animals in the control group received 0.9% NaCl solution). Carrier-free NaI/sup 131/ was given in doses of 0.05 to 10.0 mu C/g. The total iodine activity of the blood, liver, and muscle was found to be the same in the experimental as in the control group. The activity curves for both blood and muscle of mice showed essentially the same characteristics. The amount of iodine increased for the first 30 min, then decreased. After 24 hr it increased again, owing to the release of iodine-labeled hormones from the thyroid gland. The specific activity of rat serum showed similar characteristics to that of mice serum, except that the 24-hr value was higher. This observation was the subject of further experiments. The specific activity of the filtrate of rat serum taken at 24 hr notably increased, while the amount of iodine absorbed by the asbestos filter decreased as compared with the findings on mouse serum. A marked difference was found in the total activity of blood in rats and mice. Electrophoretic separation of serum proteins using 0.01 to 0.02 ml serum showed that most of the iodine in mouse serum was already protein bound after 20 hr. The distribution of the liar-labeled hormones among the serum protein was electrophoretically determined in paper strips. The activity curves obtained from radioelectropherograms of serum show the height of curves for mice to be about 1/4 those of rats. Possible causes for the observed changes in I/sup 131/ distribution are discussed, in relation to the effects of carnitine and species differences. A possible influence of radiation injury to the thyroid cannot be excluded if the high dose used (10 mu C I/sup 131/) is considered. The thyrotropic hormone activity in the anterior pituitary of the rat is markedly greater than in other animals. It is concluded that L-carnitine does not change the I/sup 131/ distribution in muscle and liver of mice. (BBB)

• Research Organization: Karl Marx Universitat, Leipzig
• NSA Number: NSA-18-011562
• OSTI ID: 4075785
• Journal Information: Acta Biologica et Medica Germanica (East Germany), Journal Name: Acta Biologica et Medica Germanica (East Germany) Vol. Vol: 9; ISSN ABMGA
• Country of Publication: Country unknown/Code not available
• Language: German

Related Subjects

BIOLOGY AND MEDICINE
BLOOD
BLOOD SERUM
DISEASES
DISTRIBUTION
DRUGS
ELECTROPHORESIS
GLANDS
HORMONES
IODINE 131
LABELLED COMPOUNDS
LIVER
METABOLISM
MICE
MUSCLES
PITUITARY GLAND
PROTEINS
QUANTITY RATIO
RADIATION DOSES
RADIATION INJURIES
RATS
THYROID
TOXICITY
TSH
VITAMIN B GROUP
VITAMIN B-T
VITAMINS