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U.S. Department of Energy
Office of Scientific and Technical Information

COMPARATIVE RESISTANCE OF STRAINS OF CLOSTRIDIUM BOTULINUM TO IONIZING RADIATION. Report No. 7 (Progress), December 11, 1959-March 10, 1960

Technical Report ·
OSTI ID:4071703

Spore suspensions of six strains each of Type-A and Type-B, and three strains of Type-E Cl. botulinum were prepared and standardized. Inocula of 100 million spores per can (202 x 202) of a beef stew substrate were exposed to various levels of gamma rays to produce partial spoilage. From the partial spoilage data, radiation D values for 90% destrnction are calculated as a measure of radiation resistance. The data previously reported for the Iwanai TypeE strain were withdrawn as invalid and results sre summarized for VH, Beluga, and 8E strains. The data now show all Type-E strains lower than the lowest Type-B strain. Procedures are boing developed for spore production of Type-C strains. However, sporulation ability ap pears to vary from strain to strain, as well as the potential viability of the stralns. These factors are currently under investigation. (auth)

Research Organization:
Continental Can Co., Inc., Chicago
NSA Number:
NSA-15-021992
OSTI ID:
4071703
Report Number(s):
AD-251633
Country of Publication:
United States
Language:
English