HIV transcription is induced with some forms of cell killing

Technical Report · Fri Nov 01 00:00:00 EST 1996

DOI:https://doi.org/10.2172/405748· OSTI ID:405748

Woloschak, G E ^[1]; Schreck, S ^[2]; Panozzo, J ^[3]; Chang-Liu, C -M ^[1]; Libertin, C R ^[3]

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1. Argonne National Lab., IL (United States)
2. Argonne National Lab., IL (United States); South Carolina Univ., Columbia, SC (United States). Dept. of Chemistry
3. Loyola Univ. Medical Center, Maywood, IL (United States)

Using HeLa cells stably transfected with an HIV-LTR-CAT construct`, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {Gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires function p53, which is missing in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States)
• Sponsoring Organization: USDOE Office of Energy Research, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 405748
• Report Number(s): ANL/CMB/PP-83769; ON: DE97001021; TRN: 97:001195
• Resource Relation: Other Information: PBD: [1996]
• Country of Publication: United States
• Language: English

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55 BIOLOGY AND MEDICINE
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TRANSCRIPTION
GENETICS
DNA REPLICATION
GENE REPRESSORS