THE CHANGES OF GERM CELLS IN X-RAY IRRADIATED MOUSE
Journal Article
·
· Ibaraki Diagaku Nogakubu Gakujtsu Hokoku
OSTI ID:4044604
Adult male mice of 3 inbred strains 2 to 3 months of age, were exposed to single x-ray doses of 150 to 5000 r. The testes were removed at varying intervals after irradiation, and the degenerative and regenerative processes of germinal epithelium were examined cytologically. The relationship between single doses of x rays and mortality in mice was found to be of a sigmoid type. Both tests and seminiferous tubules were much reduced in size on the 3Oth to 4Oth day sfter 500 r snd 1000 r irradiations, and the repairing of injured testes was observed between the 2Oth and llOth day following treatment. Based on the histological observations and breeding tests, 1000 r was sufficient to produce permanent sterility, 500 r produced temporary sterility, and 250 r was the minimum dose to give changes of the spermatogenesis. After 500 r and 1000 r irradiation, mitosis disappeared in 10 to 20 days after exposure, and most of the tubules consisted of a single layer of spermatogonia and Sertoli cells in 20 to 25 days; spermatozoa completely disappeared in 25 to 40 days. Repair of spermatogenesis began to appear in spermatogonial division at the 25th day after 500 r irradiation, and on the 45th to 6Oth day spermatozoa could be found again. In mice irradiated with 1000 r many tubules did not recover. As cytological phenomena taking place in association with the degeneration stickiness and swelling of chromosomes, chromosome bridges at anaphase of meiosis, scattering or displacement of chromosomes at metaphase, irregular distribution of chromosomes at anaphase, varying number of chromosomes, formation of bi- and multi-nucleate cells, uni- and multipolar mitoses, like lamp-brush chromosome at pachytene stage, and many aberrations of mitochondria were observed. Residual spermatogonia were poor in DNA content, and developing spermatogonia were considered to be relatively radioresistant. (Absts. Japan. Med., 1: No. 7, 1960.)
- Research Organization:
- Ibaraki Univ., Japan
- NSA Number:
- NSA-15-022031
- OSTI ID:
- 4044604
- Journal Information:
- Ibaraki Diagaku Nogakubu Gakujtsu Hokoku, Journal Name: Ibaraki Diagaku Nogakubu Gakujtsu Hokoku Vol. Vol: 5
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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