PERSISTING CHROMOSOME ABERRATIONS IN CELL CULTURES FROM IRRADIATED HUMAN SKIN
Chromosome patterns were studied in cultures in cells biopsied from skin before and after exposure to 100-r doses of 100-kv x-ray therapy. Minor numbers of chromatid aberrations were noted both in the irradiated biopsies and in the unexposed samples, and differences, whether qualitative or quantitative, could not be definitely ascertained. Various chromosome aberrations were demonstrable in the irradiated biopsies but not in the nonexposed specimens. The frequency of mitoses involving aberrations was highest in the biopsy taken 24 hr after irradiation; it was slightiy lower in the biopsy taken 3 hr after irradiation; a considerably lower frequency was seen in the biopsy taken 72 hr after irradiation. About 10% of the mitoses presented one or more aberrations. Chromosome aberrations could be classified into a few main groups. Translocation was manifest in the form of chromosomes in which one pair of arms was appreciably longer than that of the homologous chromosome, as seen in chromosome numbers 1, 2, 4-5, and 13-15 (Denver classification). Mitoses was found in which the normal numerical distributions on groups (6-12), (17-18), and (16, 19-20) was absent, indicating reciprocal translocation of the smaller chromosome. lsolated mitoses presenting similar shifts were demonstrable, however, in the unexposed biopsy, and thus these variations were not classified as aberrations. Some aberrations in the form of pericentric inversions were visible, but only on the large chromosomes. Deletion represented a common type of aberration; generally the site of fragmentation on one pair of arms was localized close to the centromere. The deleted fragments could not be traced and was perhaps lost during previous cell divisions. Development of one, and probably of several clones was noted, the same characteristic chromosome aberrations being repeated in a series of cells from various subcultures originating the same biopsy. Thus in vivo irradiation (100 r) of skin was found to induce development of chromosome aberrations persisting in cell cultures after cultivation for long periods (2 to 4 weeks). (BBB)
- Research Organization:
- Radium Center, Copenhagen
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-019563
- OSTI ID:
- 4038070
- Journal Information:
- Acta Pathologica et Microbiologica Scandinavica (Denmark) Divided into Acta Pathol. Microbiol. Scand., Sect. A and Acta Pathol. Microbiol. Scand., Sec, Vol. Vol: 59; Other Information: Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
RADIATION-INDUCED CHROMOSOME ABERRATIONS IN HUMAN FETAL CELLS GROWN IN VITRO
Persistent chromosome damage induced by localized radiotherapy for lymphoma