RADIATION-INDUCED CHROMOSOME ABERRATIONS IN HUMAN FETAL CELLS GROWN IN VITRO

Journal Article · Mon Oct 01 04:00:00 EDT 1962 · Acta Genet. Med. Gemellol. (Rome)

OSTI ID:4705411

Boeok, J A

Diploid human cells (from fetal lung and brain) grown in. vitro were irradiated with Co/sup 60/ gamma rays. The effect of radiation was measured by recording postmetaphase chromosomal aberrations in cell cultures fixed and stained 24 and 48 hr after acute irradiation. Spontaneous aberration frequencies were determined in matched control cultures. The frequency of spontaneous aberrations appeared to vary with respect to: differences between the individual fetuses from whom the biopsied cells were taken; tissue of origin whether within or between individuals; and the sex of the cultured cells. Estimates of the overall averages of spontaneous aberratlons were 0.7% for the cell cultures derived from fetal lung and 2.5% for those from fetal brain. In the irradiated cell cultures, which received doses varying from 9 to 136 rad, the number of aberrant postmetaphase figures per 100 cells/rad varied from 0.10 to 0.15 for cultures derived from lung and from 0.19 to 0.37 for cultures from brain, all at 24 hr. At 48 hr after irradiation the frequencies were somewhat lower but the same trends remained the same. Cell cultures derived from brain appear to have a higher radiosensitivity than those derived from lung. Furthermore, a sexual dimorphism was evident because, in all series of cultures, those composed of XY cells had a higher aberration frequency as compared to those with XX cells. (TCO)

• Research Organization: Uppsala Univ.
• NSA Number: NSA-17-024902
• OSTI ID: 4705411
• Report Number(s): TID-18016
• Journal Information: Acta Genet. Med. Gemellol. (Rome), Journal Name: Acta Genet. Med. Gemellol. (Rome) Vol. Vol: 11
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
BRAIN
CHROMOSOMES
COBALT 60
FETUSES
GAMMA RADIATION
LUNGS
MAN
MITOSIS
RADIATION DOSES
RADIATION EFFECTS
SEX