Radiation Injury Resulting from Nuclear Labeling with Tritiated Thymidine in the Chick Embryo
Two- to three-day old chick embryos were treated with thymidine-H/sup 3/ (10 to 50 mu c per embryo; specific activity 1.6 or 1.9 curies per millimole) through a window in the shell, and incubation was continued for 12 to 55 hours longer. All embryos incubated after treatment for 12 hours or longer displayed cytological changes characteristic of radiation injury. Injury could be avoided and satisfactory radioautographs still obtained by diluting the thymidine-H/sup 3/ one hundred to five hundred times with unlabeled thymidine, thus reducing the specific activity to 4 to 20 mu c/ mu M. (auth)
- Research Organization:
- Univ. of Texas, Medical Branch, Galveston
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-017949
- OSTI ID:
- 4030100
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 5 Vol. 14; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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