UPTAKE OF Fe$sup 59$ BY SPLEENS OF LETHALLY IRRADIATED MICE GIVEN ISOLOGOUS BONE MARROW
An approach to a study of radiation effects on hematopoietic cells, as well as in vitro protection and recovery of irradiated cells, was made in terms of their ability to transplant and proliferate in lethally irradiated mice. The system works reasonably well as a means of estimating in vitro radiosensitivity and protection of mouse bone marrow when 30-day survival of the irradiated recipients is used as an endpoint. Male (C3H x C57BL)F/sub 1/ mice were exposed to 950 r of 250-kv whole-body x rays. The next day ( approximates 24 hr) these mice were injected intravenously with graded doses of isologous bone marrow cells. Seven days later, 0.2/ mu C of /sup 56/Fe was injected intraperitoneally, and 24 hr later the spleen was removed and counted in a welltype scintillation detector. When results were expressed as percentage-uptake of injected /sup 59/Fe, there was a linear response with increasing marrow doses between 0.050 and 0.4 x 10/sup 6/ cells. Over the same range, 30-day survival of lethally irradiated mice increases from a low level to essentially l00% for many strains. It is anticipated that the method will be sensitive enough to study modification of in vitro radiation injury to marrow cells. (auth)
- Research Organization:
- Oak Ridge National Lab., Tenn.
- NSA Number:
- NSA-18-019594
- OSTI ID:
- 4024713
- Journal Information:
- Annals of the New York Academy of Sciences (U.S.), Journal Name: Annals of the New York Academy of Sciences (U.S.) Vol. Vol: 114; ISSN ANYAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
BIOLOGY AND MEDICINE
BLOOD CELLS
BLOOD VESSELS
BODY
BONE MARROW
DETECTION
DIAGRAMS
DIFFUSION
DISTRIBUTION
INTESTINE
IRON 56
IRON 59
IRRADIATION
LETHAL DOSE
MEASURED VALUES
METABOLISM
MICE
MITOSIS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RADIATION PROTECTION
RADIOSENSITIVITY
RECOVERY
REGENERATION
REPRODUCTION
SCINTILLATION COUNTERS
SPLEEN
SURVIVAL TIME
SUSPENSIONS
TISSUES
TRANSPLANTS
X RADIATION