THE FATE OF LETHALLY IRRADIATED MICE GIVEN ISOLOGOUS AND HETEROLOGOUS THORACIC DUCT LYMPHOCYTES
Lethally x irradiated (1000 rad) mice of a highly inbred stran (CBA) were injected with large numbers of isologous thoacic duct cells. The survival time of the animals was recorded and their tissues were examined for the presence of poliferating marrow cells. Intravenous injection of 8 to 18.8 x 107 lymphocytes did not reduce the mortality from radiation, nor did it lead to any hematopoiesis in spleen or bone marrow. However, it restored the ability of lethally irradiated mice to reject a graft of rat bone marrow. Lethally irradiated mice which received rat thoracic duct lymphocytes died earlier than untreated controls or mice receiving isologous lymphocytes. An injection of rat lymphocytes also eliminated the beneficial effect of treatment with isologous bone marrox. These reactions of lethally irradiated mice were accompanied by the appearance and proliferation in the splenic white-pulp of large cells with an intensely pyroninophilic cytoplasm, a pale nucleus, and a prominent nucleolus. These cells probably developed from the injected lymphocytes. The experiments do not support the idea that lymphocytes can transform into marrow stem cells, but they illustrate the immunological activity of lymphocytes. These findings are at variance with those of Delorme (Lancet, 2: 855(1961)), who found that isologous thoracic duct cells protected 43% of rats against a lethal dose of x irradiation. This discrepancy is probably due to differences in the sensitivity of mouse and rat tissues to x irradiation. (H.H.D.)
- Research Organization:
- Sir William Dunn School of Pathology, Oxford
- NSA Number:
- NSA-17-001267
- OSTI ID:
- 4773678
- Journal Information:
- Brit. J. Exptl. Pathol., Journal Name: Brit. J. Exptl. Pathol. Vol. Vol: 43
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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