EFFECT OF X-IRRADIATION ON BLOOD CELLS AND TISSUES. Final Report for Period October 1, 1952-June 30, 1961. Report 513(Final)

Technical Report · Thu Jun 01 04:00:00 EDT 1961

OSTI ID:4024492

Lessler, M A

A review is given of significant findings on the effect of x-irradiation on the cytophysiology of blood cells. Evidence of cytological, histochemical, and biophysical changes are related to changes in respiratory activity of frog and amphiuma red cells noted following x-ray doses of 100 and 200 r in vitro. Recent studies of x-ray damage to frog blood cells at the electron microscope level of resolution are presented. The studies indicate that a dose of 1000 rads results in the disruption of the ultra-structure of red cell mitochondria and in the appearance of vacuoles in the cytoplasm of leukocytes. The metabolism of freshly prepared and blood-bank stored human erythrocytes incubated in autologous plasma or physiological saline solution was compared. Erythrocyte respiration and glycolysis was greater in plasma than in saline. Glycolysis of unirradiated and 700 r x-irradiated red cells was approximately equal in the normal range of blood glucose levels, but at higher or lower glucose levels in vitro x- irradiation inhibited glycolysis. Exposure of blood-bank stored erythrocytes to x-ray doses of 700 or 1400 r did not materially alter their respiratory activity. A comparison of the endogenous respiration of rat liver and spleen slices after in vitro x-irradiation of 300 and 1200 r is reported. (auth)

• Research Organization: Ohio State Univ. Research Foundation, Columbus
• NSA Number: NSA-15-024677
• OSTI ID: 4024492
• Report Number(s): NP-10372
• Country of Publication: United States
• Language: English

Related Subjects

ANIMALS
BIOLOGY AND MEDICINE
BLOOD PLASMA
ELECTRON MICROSCOPY
ENZYMES
ERYTHROCYTES
GLANDS
GLUCOSE
LEUCOCYTES
LIVER
MITOCHONDRIA
RADIATION DOSES
RADIATION EFFECTS
RATS
SPLEEN
SUGARS
TISSUES
X RADIATION