SURVIVAL KINETICS OF HeLa S-3 CELLS AFTER INCORPORATION OF $sup 3$H- THYMIDINE OR $sup 3$H-URIDINE

Journal Article · Sun Sep 01 04:00:00 EDT 1963 · Intern. J. Radiation Biol.

OSTI ID:4023992

Marin, G; Bender, M A

The survival kinetics of mammalian tissue culture cells containing tritium-labeled nucleosides were studied. He La S-3 cells were allowed to incorporate various amounts of /sup 3/H-thymidine or /sup 3/H-uridine into their DNA or RNA. Owing to the short mean free path of the tritium-decay BETA -ray, incorporation of /sup 3/H-thymidine irratliates mainly the nucleus, while most of the radiation from the /sup 3/H-uridine is absorbed by the cytoplasm. A model which takes into account the persistence of BETA radiation from the labeled nucleic acids and the dose rate reduction due to cell division was constructed for the expected survival kinetics. The experimental data were fitted to this model by means of a digital computer. It was found that the hit number was not significantly different from the values previously reported for x rays. It was concluded that all the cell killing seen can be accounted for by the BETA radiation to which the cell is exposed. (auth)

• Research Organization: Oak Ridge National Lab., Tenn.
• NSA Number: NSA-18-019644
• OSTI ID: 4023992
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 7
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BETA DECAY
BIOLOGY AND MEDICINE
CELL NUCLEI
COMPUTERS
DIGITAL SYSTEMS
DNA
HELA CELLS
HETEROCYCLICS
LABELLED COMPOUNDS
METABOLISM
MITOSIS
NUCLEIC ACIDS
NUCLEOSIDES
ORGANIC NITROGEN COMPOUNDS
RIBONUCLEIC ACID
SURVIVAL TIME
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM
URIDINE
VARIATIONS
X RADIATION