A COMPARISON OF MAMMALIAN CELL-KILLING BY INCORPORATED $sup 3$H-THYMIDINE AND $sup 3$H-URIDINE

Journal Article · Sun Sep 01 04:00:00 EDT 1963 · Intern. J. Radiation Biol.

OSTI ID:4037269

Marin, G; Bender, M A

The fact that the BETA radiation from /sup 3/H atoms incorporated into cellular DNA is absorbed largely in the nucleus, while more of that from /sup 3/H incorporated into RNA is absorbed in the cytoplasm than in the nucleus, provides a means of determiring the relative importance of nuclear and cytoplasmic damage in radioinduced mammalian cell death. Synchronously dividing populntions of Chinese hamster fibroblasts were pulse-labeled with various doses of either /sup 3/H-thymidine or /sup 3/H-uridine. The labeled cells were plated, and survival curves obtained. Autoradiographic silver grain counts and liquid scirtillation counting were used to measure the nuclear BETA radiation doses. It was found that the slope of the /sup 3/H-uridine survival curve was somewhat greater than that of the /sup 3/H-thymidine curve. Since the BETA radiation dose to the cytoplasm was larger than that to the nucleus, however, it is concluded that the contribution of cytoplasmic damage to radioinduced reproductive death of mammalian ceils is very small. (auth)

• Research Organization: Oak Ridge National Lab., Tenn.
• NSA Number: NSA-18-019645
• OSTI ID: 4037269
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 7
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ABSORPTION
ANIMAL CELLS
BETA DECAY
BIOLOGY AND MEDICINE
CELL NUCLEI
CYTOLOGY
DIAGRAMS
DNA
HETEROCYCLICS
LABELLED COMPOUNDS
MEASURED VALUES
METABOLISM
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PERFORMANCE
RADIATION EFFECTS
RADIATION INJURIES
RADIOACTIVITY
RADIOAUTOGRAPHY
RIBONUCLEIC ACID
SCINTILLATION COUNTERS
SURVIVAL TIME
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM
URIDINE
VARIATIONS