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SYNTHESIS: BIOLOGICAL HALF-LIFE, ELECTROPHORETIC PATTERNS, AND CONCENTRATION OF RAT SERUM PROTEINS AFTER X-IRRADIATION

Thesis/Dissertation ·
OSTI ID:4022483

The changes in concentration of various serum protein fractions in rats after 3000 of whole-body x irradiation were studied. Pre- and post-treatment serum samples from the same animal were separated by paper strip electrophoresis. The ratios of% post- to% pre-treatment values were compared for starved controls, fed controls, irradiated animals, spleen-shielded animals, liver-shielded animals, and spleen and liver-shielded animals. (A percentage figure refers to the % of total serum protein found in any particular fraction). The data indicated a disturbance in synthesis following irradiation but did not exclude an alteration in biological haif life of the various fractions. The incorporation of /sup 35/-labeled L-methionine into serum proteins was studied using 12 starved rats as controls and 12 whole-body-irradiated rats. The data indicated an increase in synthesis rates when serum protein fraction concentrations were increased ( alpha /sub 1/-, alpha /sub 2/-, and BETA -giobulins) and a decrease in synthesis rates when serum protein fractions were decreased ( gamma -globulin and albumin). Additional experiments indicated that the biological half lives of the various serum protein fractions were not altered by irradiation. (Dissertation Abstr., 24: No. 8, Feb. 1964)

Research Organization:
Originating Research Org. not identified
NSA Number:
NSA-18-019754
OSTI ID:
4022483
Country of Publication:
Country unknown/Code not available
Language:
English