Transfer of adoptive immunity to tuberculosis in mice

Journal Article · Sun Jun 01 04:00:00 EDT 1975 · Infect. Immun., v. 11, no. 6, pp. 1174-1181

OSTI ID:4021023

Lefford, M J

A system is described for studying adoptive immunity to tuberculosis in syngeneic mice. Donor mice were immunized with 10$sup 4$ BCG intravenously, and lymphoid cells were harvested 28 days later. Adoptive immunity was measured in recipient mice in terms of the inhibition of growth of BCG in the liver and spleen following intravenous injection. Adoptive immunity was expressed optimally when recipients were sublethally irradiated (500 R), challenged with 10$sup 4$ to 10$sup 5$ viable organisms, and given sensitized lymphoid cells intravenously. Adoptive immunity was not manifest until 14 days after challenge and was effective against Mycobacterium tuberculosis H37Rv as well as BCG. Immunity could be conferred by spleen, lymph node, peritoneal exudate, and resident peritoneal (washout) cells. The lymphoid cells conferring immunity were shown to be thymus-dependent lymphocytes by virtue of their nonadherence to glass wool and sensitivity to anti-theta serum plus complement. The sensitized cells were relatively susceptible to both in vitro and in vivo x irradiation. (auth)

• Research Organization: Trudeau Inst., Saranac Lake, NY
• NSA Number: NSA-33-026272
• OSTI ID: 4021023
• Journal Information: Infect. Immun., v. 11, no. 6, pp. 1174-1181, Journal Name: Infect. Immun., v. 11, no. 6, pp. 1174-1181; ISSN INFIB
• Country of Publication: United States
• Language: English

Related Subjects

*IMMUNE REACTIONS-- BIOLOGICAL RADIATION EFFECTS
*TUBERCULOSIS-- IMMUNITY
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SUBLETHAL IRRADIATION
WHOLE-BODY IRRADIATION
X RADIATION