Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea

Journal Article · Mon Mar 01 04:00:00 EST 1976 · Blood, v. 47, no. 3, pp. 403-411

OSTI ID:4016282

Broxmeyer, H E; Galbraith, P R; Baker, F L

Suspensions of human bone marrow cells were subjected to $sup 137$Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony-stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony-forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA. (auth)

• Research Organization: Queen's Univ., Kingston, Ont.
• NSA Number: NSA-33-026243
• OSTI ID: 4016282
• Journal Information: Blood, v. 47, no. 3, pp. 403-411, Journal Name: Blood, v. 47, no. 3, pp. 403-411; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

*CFU-- BIOLOGICAL RADIATION EFFECTS
*HYDROXY COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*UREA-- RADIOSENSITIVITY EFFECTS
560151* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Man
BONE MARROW CELLS
CESIUM 137
GAMMA RADIATION
IN VITRO
IRRADIATION
MAN
N48510* --Life Sciences--Radiation Effects on Animals--Man