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Title: STUDIES ON THE DISTRIBUTION AND PHOSPHATE TURNOVER OF THE ACID-SOLUBLE PHOSPHORUS COMPOUNDS IN VARIOUS NORMAL AND NEOPLASTIC TISSUES OF RATS. I. A SEMI-MICRO GRADIENT ELUTION CHROMATOGRAPHY WITH DOWEX FORMATE AND IDENTIFICATION OF SOME PHOSPHATE ESTERS ON THE CHROMATOGRAM (ENGLISH TEXT)

Journal Article · · Journal of Biochemistry (Tokyo) (Japan)
OSTI ID:4011448

A modified semi-micro gradient elution chromatography for the analysis of tissue acid-soluble phosphorus compounds is described. One to 3 g of tissue can be analyzed by this method. Liver, muscle, and thymus tissue of rats were analyzed and the chromatograms are illustrated. The distribution and turnover of the acid-soluble phosphorus compounds in rat liver were also studied by P/sup 32/ injection and the use of the semi-micro method. Glucose 6-phosphate and L- alpha -glycerophosphate were identified on the chromatogram, and a phosphorus compound containing aminoacid was separated from the dowex 1 formate non-adsorbable fraction. (Abstr. Japan Med., 1: No. 9, 1961)

Research Organization:
Tokyo Univ.
NSA Number:
NSA-15-025840
OSTI ID:
4011448
Journal Information:
Journal of Biochemistry (Tokyo) (Japan), Vol. Vol: 47; Other Information: Orig. Receipt Date: 31-DEC-61
Country of Publication:
Country unknown/Code not available
Language:
English