Genetic toxicity evaluation of 1,1,1,2,3,3,3- heptatfluoropropane. Volume 1. Results of salmonella typhimurium histidine reversion assay (ames assay). Final report, March-December 1994
Under subcontract to ManTech Environmental Technology, Incorporated, Uenesys Research, Incorporated tested 1,1,12,3,3,3- heptafluoropropane (HFC-227ea) using Billups-Rothenberg exposure chambers for the exposure chamber modification of the Salmonella typhimurium histidine (his) reversion mutagenesis system (the Ames test), a microbial assay that measures his his+ reversion induced by chemicals that cause base changes or frameshift mutations i the genome of this organism. Testing was conducted using five Salmonella strains, with and without metabolic activation. HFC-227ea was tested in a preliminary test and in a mutagenesis assay. HFC-227ea was tested to toxic levels in the mutagenesis assay, but a sufficient number of nontoxic concentrations were tested to determine if HFC-227ea were capable of inducing a dose-related mutagenic response, and the positive control responses were consistent with historical data from the laboratory, and no evidence of a mutagenic response was obtained in any strain without or with activation. Therefore, HFC-227ea was negative in the Salmonella typhimurium histidine reversion mutagenesis test in the presence and absence of metabolic activation.
- Research Organization:
- ManTech Environmental Technology, Inc., Research Triangle Park, NC (United States). Toxic Hazard Research Unit
- OSTI ID:
- 370980
- Report Number(s):
- AD-A--308301/1/XAB; CNN: Contract F33615-90-C-0532
- Country of Publication:
- United States
- Language:
- English
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