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Effects of halides on plasmid-mediated silver resistance in Escherichia coli

Journal Article · · Applied and Environmental Microbiology
OSTI ID:302311
; ;  [1]
  1. Univ. of Illinois, Chicago, IL (United States). Dept. of Microbiology and Immunology
Silver resistance of sensitive Escherichia coli J53 and resistance plasmid-containing J53(pMG101) was affected by halides in the growth medium. The effects of halides on Ag{sup +} resistance were measured with AgNO{sub 3} and silver sulfadiazine, both on agar and in liquid. Low concentrations of chloride made the differences in MICs between sensitive and resistant strains larger. High concentrations of halides increased the sensitivities of both strains to Ag{sup +}. The purpose of this report is to set out easy-to-use conditions for measuring silver sensitivity and resistance in familiar and widely used media, Luria-Bertani (LB) agar and broth, so as to facilitate wider identification of silver resistance in nature.
Sponsoring Organization:
USDOE, Washington, DC (United States)
OSTI ID:
302311
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 12 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
Country of Publication:
United States
Language:
English

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