Cr{sup 6+} sensitivity of Pseudomonas fluorescens transposon mutants - 15725
- Department of Biology, Claflin University Orangeburg, SC 29115 (United States)
Bacteria have evolved systems for heavy metal resistance and reduction that can be exploited for bioremediation. This pilot research project looks at the reduction of chromium present at DOE's Savannah River Site and the ability of the bacterial population to aid in the reduction of heavy metals. The research goal was to determine the genes responsible for chromium resistance in a Pseudomonas fluorescens Pf5 isolate capable of Cr(VI) reduction using transposon mutagenesis. P. fluorescens was mated with Escherichia coli containing pTnModRKm' which has a mini-Tn5 transposon. The ex-conjugates were plated on Luria Bertani agar with kanamycin. Individual colonies were then replica plated onto LB agar with 25 ppm of potassium dichromate. One mutant out of ∼8000 mutants was found to have increased sensitivity to increased sensitivity to Cr{sup 6+}. The mutant will be examined for chromium reduction and the mutated gene will be determined. (authors)
- Research Organization:
- WM Symposia, Inc., PO Box 27646, 85285-7646 Tempe, AZ (United States)
- OSTI ID:
- 22824535
- Report Number(s):
- INIS-US--19-WM-15725
- Country of Publication:
- United States
- Language:
- English
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