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Title: A method for measuring bacterial chemotaxis parameters in a microcapillary

Abstract

A new method was developed which enables chemotaxis parameters to be measured at a single-cell level inside a capillary for the first time. The chemotaxis chamber consists of two reservoirs communicating through a capillary tube 50 {micro}m in diameter. Chemotaxis parameters are measured inside the capillary using image analysis, after a nearly linear attractant concentration gradient has been generated along the capillary by diffusion. Compared to previously published techniques, this method provides a well-characterized chemoattractant concentration profile in addition to allowing single-cell parameters to be measured inside a fine capillary. This procedure was used to measure the single-cell chemotaxis parameters for Escherichia coli K12, and the results are compared to published data on single E. coli cells chemotaxing in bulk.

Authors:
;  [1]
  1. Tulane Univ., New Orleans, LA (United States). Dept. of Chemical Engineering
Publication Date:
OSTI Identifier:
260443
DOE Contract Number:
FG21-93EW53023
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnology and Bioengineering; Journal Volume: 51; Journal Issue: 1; Other Information: PBD: 5 Jul 1996
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; ESCHERICHIA COLI; BEHAVIOR; REMEDIAL ACTION; MEASURING METHODS; STIMULI; BIODEGRADATION; WASTE PROCESSING

Citation Formats

Liu, Z., and Papadopoulos, K.D.. A method for measuring bacterial chemotaxis parameters in a microcapillary. United States: N. p., 1996. Web. doi:10.1002/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.3.CO;2-D.
Liu, Z., & Papadopoulos, K.D.. A method for measuring bacterial chemotaxis parameters in a microcapillary. United States. doi:10.1002/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.3.CO;2-D.
Liu, Z., and Papadopoulos, K.D.. Fri . "A method for measuring bacterial chemotaxis parameters in a microcapillary". United States. doi:10.1002/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.3.CO;2-D.
@article{osti_260443,
title = {A method for measuring bacterial chemotaxis parameters in a microcapillary},
author = {Liu, Z. and Papadopoulos, K.D.},
abstractNote = {A new method was developed which enables chemotaxis parameters to be measured at a single-cell level inside a capillary for the first time. The chemotaxis chamber consists of two reservoirs communicating through a capillary tube 50 {micro}m in diameter. Chemotaxis parameters are measured inside the capillary using image analysis, after a nearly linear attractant concentration gradient has been generated along the capillary by diffusion. Compared to previously published techniques, this method provides a well-characterized chemoattractant concentration profile in addition to allowing single-cell parameters to be measured inside a fine capillary. This procedure was used to measure the single-cell chemotaxis parameters for Escherichia coli K12, and the results are compared to published data on single E. coli cells chemotaxing in bulk.},
doi = {10.1002/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.3.CO;2-D},
journal = {Biotechnology and Bioengineering},
number = 1,
volume = 51,
place = {United States},
year = {Fri Jul 05 00:00:00 EDT 1996},
month = {Fri Jul 05 00:00:00 EDT 1996}
}