Comparative genomics of Geobacter chemotaxis genes reveals diverse signaling function

Tran, Hoa T.; Krushkal, Julia; Antommattei, Frances M.; Lovley, Derek R.; Weis, Robert M.

doi:10.1186/1471-2164-9-471

Comparative genomics of Geobacter chemotaxis genes reveals diverse signaling function

Journal Article · Mon Dec 31 23:00:00 EST 2007 · BMC Genomics

DOI:https://doi.org/10.1186/1471-2164-9-471· OSTI ID:1626470

Tran, Hoa T. ^[1]; Krushkal, Julia ^[2]; Antommattei, Frances M. ^[3]; Lovley, Derek R. ^[4]; Weis, Robert M. ^[5]

Univ. of Massachusetts, Amherst, MA (United States). Dept. of Chemistry; DOE/OSTI
Univ. of Tennessee Health Science Center, Memphis, TN (United States). Dept. of Preventive Medicine
Univ. of Massachusetts, Amherst, MA (United States). Dept. of Chemistry; Procter and Gamble, Cincinnati, OH (United States). Sharon Woods Technical Center
Univ. of Massachusetts, Amherst, MA (United States). Dept. of Microbiology
Univ. of Massachusetts, Amherst, MA (United States). Dept. of Chemistry

Background: Geobacter species are δ-Proteobacteria and are often the predominant species in a variety of sedimentary environments where Fe(III) reduction is important. Their ability to remediate contaminated environments and produce electricity makes them attractive for further study. Cell motility, biofilm formation, and type IV pili all appear important for the growth of Geobacter in changing environments and for electricity production. Recent studies in other bacteria have demonstrated that signaling pathways homologous to the paradigm established for Escherichia coli chemotaxis can regulate type IV pili-dependent motility, the synthesis of flagella and type IV pili, the production of extracellular matrix material, and biofilm formation. The classification of these pathways by comparative genomics improves the ability to understand how Geobacter thrives in natural environments and better their use in microbial fuel cells. Results: The genomes of G. sulfurreducens, G. metallireducens, and G. uraniireducens contain multiple (~70) homologs of chemotaxis genes arranged in several major clusters (six, seven, and seven, respectively). Unlike the single gene cluster of E. coli, the Geobacter clusters are not all located near the flagellar genes. The probable functions of some Geobacter clusters are assignable by homology to known pathways; others appear to be unique to the Geobacter sp. and contain genes of unknown function. We identified large numbers of methyl-accepting chemotaxis protein (MCP) homologs that have diverse sensing domain architectures and generate a potential for sensing a great variety of environmental signals. We discuss mechanisms for class-specific segregation of the MCPs in the cell membrane, which serve to maintain pathway specificity and diminish crosstalk. Finally, the regulation of gene expression in Geobacter differs from E. coli. The sequences of predicted promoter elements suggest that the alternative sigma factors σ28 and σ54 play a role in regulating the Geobacter chemotaxis gene expression. Conclusion: The numerous chemoreceptors and chemotaxis-like gene clusters of Geobacter appear to be responsible for a diverse set of signaling functions in addition to chemotaxis, including gene regulation and biofilm formation, through functionally and spatially distinct signaling pathways.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Massachusetts, Amherst, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FC02-02ER63446

OSTI ID:: 1626470

Journal Information:: BMC Genomics, Journal Name: BMC Genomics Journal Issue: 1 Vol. 9; ISSN 1471-2164

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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