Highly Active Carbon–Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application

Kafil, Vala; Sreenan, Benjamin; Ra Shin, Sun Hae; Brennan, Alec A.; Brett, Paul J.; AuCoin, David P.; Thallapally, Praveen K.; Tal-Gan, Yftah; Zhu, Xiaoshan

doi:10.1021/acsanm.4c05224

Highly Active Carbon–Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application

Journal Article · Wed Dec 11 00:00:00 EST 2024 · ACS Applied Nano Materials

DOI:https://doi.org/10.1021/acsanm.4c05224· OSTI ID:2572773

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Univ. of Nevada, Reno, NV (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Univ. of Nevada, Reno, NV (United States); Mahidol University, Bangkok (Thailand)

Nanozymes (nanomaterials with intrinsic enzyme-like characteristics) have gained much attention for diagnostics and therapy due to their excellent enzyme-mimicking capability, great stability in environments, and facile and low-cost production. However, developing nanozymes with a high catalytic constant, K_cat, has been challenging. Herein, we report a class of nanozyme-mimicking peroxidases, which are formed by depositing ultrasmall platinum nanoparticles (Pt NPs) 1–2 nm in size on the surface of hydrophilic nitrogen-doped carbon nanoparticles (CN NPs). These nanozymes defined as CN-Pt NPs show a high peroxidase-like activity with K_cat values of 1.27 M·mL/s·g for 3,3,5,5′-tetramethylbenzidine (TMB) and 1.97 M·mL/s·g for hydrogen peroxide (H₂O₂), respectively, which are at least one or two orders higher than many other reported carbon–noble metal-based nanozymes. Our developed CN-Pt NPs were further utilized in a colorimetric immunoassay as signal amplifiers for the biomarker detection of Burkholderia pseudomallei, a Gram-negative bacterial pathogen classified as a tier 1 select agent by the US CDC. In conclusion, the assay achieved lower limits of detection of 0.11 ng/mL in phosphate-buffered saline (PBS) and 0.16 ng/mL in human serum, when compared to many other assays in detecting the same biomarker.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Aeronautics and Space Administration (NASA); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division (MSE)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2572773

Report Number(s):: PNNL-SA--202949

Journal Information:: ACS Applied Nano Materials, Journal Name: ACS Applied Nano Materials Journal Issue: 24 Vol. 7; ISSN 2574-0970

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Assays
Biopolymers
Burkholderia pseudomallei
CN-Pt nanoparticle
Immunology
Peptides and proteins
Platinum
bioconjugation
capsular polysaccharides (CPS)
immunoassay
nanozyme

Highly Active Carbon–Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application

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References (54)

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