Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of A β 1-40: A Biomarker of Alzheimer’s Disease

Lyu, Zhaoyuan; Ding, Shichao; Zhang, Nan; Zhou, Yang; Cheng, Nan; Wang, Maoyu; Xu, Mingjie; Feng, Zhenxing; Niu, Xiangheng; Cheng, Yuan; Zhang, Chao; Du, Dan; Lin, Yuehe

doi:10.34133/2020/4724505

Title: Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of A β 1-40: A Biomarker of Alzheimer’s Disease

Journal Article · Mon Oct 19 00:00:00 EDT 2020 · Research

DOI:https://doi.org/10.34133/2020/4724505· OSTI ID:1691526

Lyu, Zhaoyuan ^[1];

^[1]; Zhang, Nan ^[2]; Zhou, Yang ^[1]; Cheng, Nan ^[1]; Wang, Maoyu ^[3]; Xu, Mingjie ^[4];

^[3]; Niu, Xiangheng ^[1];

^[2]; Zhang, Chao ^[5];

^[1];

^[1]

Washington State Univ., Pullman, WA (United States)
Inst. of High Performance Computing (Singapore)
Oregon State Univ., Corvallis, OR (United States)
Univ. of California, Irvine, CA (United States)
National Univ. of Singapore (Singapore)

Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-N_x SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Results indicate that the Fe-N_x SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP, owing to the maximized utilization of Fe atoms and their abundant active sites, which could mimic natural metalloproteases structures. Further designed SAN-linked immunosorbent assay (SAN-LISA) demonstrates the ultralow limit of detection (LOD) of 0.88 pg/mL, much more sensitive than that of commercial ELISA (9.98 pg/mL). The results confirm that the Fe-N_x SANs can serve as a satisfactory replacement of enzyme labels, which show great potential as an ultrasensitive colorimetric immunoassay.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1691526

Journal Information:: Research, Vol. 2020, Issue 10; ISSN 2639-5274

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (48)

Amyloid-β protein dimers isolated directly from Alzheimer's brains impair synaptic plasticity and memory Shankar, Ganesh M.; Li, Shaomin; Mehta, Tapan H. Nature Medicine, Vol. 14, Issue 8 https://doi.org/10.1038/nm1782	journal	June 2008
Cascade Reaction System Integrating Single-Atom Nanozymes with Abundant Cu Sites for Enhanced Biosensing Wu, Yu; Wu, Jiabin; Jiao, Lei Analytical Chemistry, Vol. 92, Issue 4 https://doi.org/10.1021/acs.analchem.9b05437	journal	January 2020
Dual-Quenching Electrochemiluminescence Strategy Based on Three-Dimensional Metal–Organic Frameworks for Ultrasensitive Detection of Amyloid-β Zhao, Guanhui; Wang, Yaoguang; Li, Xiaojian Analytical Chemistry, Vol. 91, Issue 3 https://doi.org/10.1021/acs.analchem.8b04332	journal	January 2019
Microbiosensor for Alzheimer’s disease diagnostics: detection of amyloid beta biomarkers: Biosensor for Abeta detection Prabhulkar, Shradha; Piatyszek, Rudolph; Cirrito, John R. Journal of Neurochemistry, Vol. 122, Issue 2 https://doi.org/10.1111/j.1471-4159.2012.07709.x	journal	April 2012
Atomically Isolated Iron Atom Anchored on Carbon Nanotubes for Oxygen Reduction Reaction Liu, Dong; Li, Jin-Cheng; Shi, Qiurong ACS Applied Materials & Interfaces, Vol. 11, Issue 43 https://doi.org/10.1021/acsami.9b12054	journal	September 2019
Fe–N–C Single-Atom Nanozymes for the Intracellular Hydrogen Peroxide Detection Jiao, Lei; Xu, Weiqing; Yan, Hongye Analytical Chemistry, Vol. 91, Issue 18 https://doi.org/10.1021/acs.analchem.9b02901	journal	August 2019
Single‐Atom Nanozyme Based on Nanoengineered Fe–N–C Catalyst with Superior Peroxidase‐Like Activity for Ultrasensitive Bioassays Cheng, Nan; Li, Jin‐Cheng; Liu, Dong Small, Vol. 15, Issue 48 https://doi.org/10.1002/smll.201901485	journal	May 2019
Validation and accuracy of peak synthesis software for XPS Seah, M. P.; Brown, M. T. Applied Surface Science, Vol. 144-145 https://doi.org/10.1016/s0169-4332(98)00787-9	journal	April 1999
Single-Atom Catalysts for Electrochemical Water Splitting Zhu, Chengzhou; Shi, Qiurong; Feng, Shuo ACS Energy Letters, Vol. 3, Issue 7 https://doi.org/10.1021/acsenergylett.8b00640	journal	June 2018
A Single‐Atom Nanozyme for Wound Disinfection Applications Xu, Bolong; Wang, Hui; Wang, Weiwei Angewandte Chemie International Edition, Vol. 58, Issue 15 https://doi.org/10.1002/anie.201813994	journal	April 2019
Bioinspired Synthesis of All-in-One Organic-Inorganic Hybrid Nanoflowers Combined with a Handheld pH Meter for On-Site Detection of Food Pathogen Ye, Ranfeng; Zhu, Chengzhou; Song, Yang Small, Vol. 12, Issue 23 https://doi.org/10.1002/smll.201600273	journal	April 2016
Alzheimer′s disease biomarkers detection in human samples by efficient capturing through porous magnetic microspheres and labelling with electrocatalytic gold nanoparticles de la Escosura-Muñiz, Alfredo; Plichta, Zdeněk; Horák, Daniel Biosensors and Bioelectronics, Vol. 67 https://doi.org/10.1016/j.bios.2014.07.086	journal	May 2015
Oxidase‐Like Fe‐N‐C Single‐Atom Nanozymes for the Detection of Acetylcholinesterase Activity Wu, Yu; Jiao, Lei; Luo, Xin Small, Vol. 15, Issue 43 https://doi.org/10.1002/smll.201903108	journal	September 2019
When Nanozymes Meet Single‐Atom Catalysis Jiao, Lei; Yan, Hongye; Wu, Yu Angewandte Chemie, Vol. 132, Issue 7 https://doi.org/10.1002/ange.201905645	journal	October 2019
Regenerable and Simultaneous Surface Plasmon Resonance Detection of Aβ(1−40) and Aβ(1−42) Peptides in Cerebrospinal Fluids with Signal Amplification by Streptavidin Conjugated to an N-Terminus-Specific Antibody Xia, Ning; Liu, Lin; Harrington, Michael G. Analytical Chemistry, Vol. 82, Issue 24 https://doi.org/10.1021/ac102257m	journal	December 2010
A Graphene Oxide˙Streptavidin Complex for Biorecognition - Towards Affinity Purification Liu, Zunfeng; Jiang, Linhua; Galli, Federica Advanced Functional Materials, Vol. 20, Issue 17 https://doi.org/10.1002/adfm.201000761	journal	July 2010
Single-Atom Electrocatalysts Zhu, Chengzhou; Fu, Shaofang; Shi, Qiurong Angewandte Chemie International Edition, Vol. 56, Issue 45 https://doi.org/10.1002/anie.201703864	journal	October 2017
An Enhanced Platform to Analyse Low-Affinity Amyloid β Protein by Integration of Electrical Detection and Preconcentrator Yoo, Yong Kyoung; Yoon, Dae Sung; Kim, Gangeun Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-14338-4	journal	October 2017
Modified screen printed electrode for development of a highly sensitive label-free impedimetric immunosensor to detect amyloid beta peptides Lien, Truong T. N.; Takamura, Yuzuru; Tamiya, Eiichi Analytica Chimica Acta, Vol. 892 https://doi.org/10.1016/j.aca.2015.08.036	journal	September 2015
Preparation of surface-imprinted microspheres using ionic liquids as novel cross-linker for recognizing an immunostimulating peptide Ding, Shichao; Hu, Xiaoling; Guan, Ping Journal of Materials Science, Vol. 52, Issue 13 https://doi.org/10.1007/s10853-017-1005-x	journal	March 2017
Leaching of manganese ores using sawdust as a reductant Hariprasad, D.; Dash, B.; Ghosh, M. K. Minerals Engineering, Vol. 20, Issue 14 https://doi.org/10.1016/j.mineng.2007.07.013	journal	November 2007
Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer Fu, Guanglei; Sanjay, Sharma T.; Dou, Maowei Nanoscale, Vol. 8, Issue 10 https://doi.org/10.1039/C5NR09051B	journal	January 2016
A reusable PMMA/paper hybrid plug-and-play microfluidic device for an ultrasensitive immunoassay with a wide dynamic range Sanjay, Sharma T.; Li, Meihan; Zhou, Wan Microsystems & Nanoengineering, Vol. 6, Issue 1 https://doi.org/10.1038/s41378-020-0143-5	journal	June 2020
Highly Efficient Oxygen Reduction Reaction Electrocatalysts Synthesized under Nanospace Confinement of Metal–Organic Framework Guo, Jianing; Li, Yang; Cheng, Yuanhui ACS Nano, Vol. 11, Issue 8 https://doi.org/10.1021/acsnano.7b03807	journal	July 2017
Mesoporous Metal–Nitrogen-Doped Carbon Electrocatalysts for Highly Efficient Oxygen Reduction Reaction Liang, Hai-Wei; Wei, Wei; Wu, Zhong-Shuai Journal of the American Chemical Society, Vol. 135, Issue 43 https://doi.org/10.1021/ja407552k	journal	October 2013
A Rapid Label-Free Electrochemical Detection and Kinetic Study of Alzheimer's Amyloid Beta Aggregation Vestergaard, Mun'delanji; Kerman, Kagan; Saito, Masato Journal of the American Chemical Society, Vol. 127, Issue 34 https://doi.org/10.1021/ja052522q	journal	August 2005
Nanocatalytic Tumor Therapy by Single-Atom Catalysts Huo, Minfeng; Wang, Liying; Wang, Youwei ACS Nano https://doi.org/10.1021/acsnano.9b00457	journal	February 2019
Single-Atom Pd ₁ /Graphene Catalyst Achieved by Atomic Layer Deposition: Remarkable Performance in Selective Hydrogenation of 1,3-Butadiene Yan, Huan; Cheng, Hao; Yi, Hong Journal of the American Chemical Society, Vol. 137, Issue 33 https://doi.org/10.1021/jacs.5b06485	journal	August 2015
Sparks fly between ascorbic acid and iron-based nanozymes: A study on Prussian blue nanoparticles Zhang, Wei; Wu, Yang; Dong, Hai-Jiao Colloids and Surfaces B: Biointerfaces, Vol. 163 https://doi.org/10.1016/j.colsurfb.2018.01.010	journal	March 2018
Multifunctional streptavidin–biotin conjugates with precise stoichiometries Xu, Dongdong; Wegner, Seraphine V. Chemical Science, Vol. 11, Issue 17 https://doi.org/10.1039/d0sc01589j	journal	January 2020
Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes Wei, Hui; Wang, Erkang Chemical Society Reviews, Vol. 42, Issue 14 https://doi.org/10.1039/c3cs35486e	journal	January 2013
Hierarchical manganese dioxide nanoflowers enable accurate ratiometric fluorescence enzyme-linked immunosorbent assay Jiao, Lei; Zhang, Lianhua; Du, Wenwen Nanoscale, Vol. 10, Issue 46 https://doi.org/10.1039/C8NR07096B	journal	January 2018
Confined Catalysis: Progress and Prospects in Energy Conversion (Adv. Energy Mater. 40/2019) Shifa, Tofik Ahmed; Vomiero, Alberto Advanced Energy Materials, Vol. 9, Issue 40 https://doi.org/10.1002/aenm.201970158	journal	September 2019
Self-Assembly of All-Inclusive Allochroic Nanoparticles for the Improved ELISA Jiao, Lei; Yan, Hongye; Xu, Weiqing Analytical Chemistry, Vol. 91, Issue 13 https://doi.org/10.1021/acs.analchem.9b01527	journal	May 2019
Single-atom catalysis of CO oxidation using Pt1/FeOx Qiao, Botao; Wang, Aiqin; Yang, Xiaofeng Nature Chemistry, Vol. 3, Issue 8 https://doi.org/10.1038/nchem.1095	journal	July 2011
Amyloid β-Protein Assembly and Alzheimer Disease Roychaudhuri, Robin; Yang, Mingfeng; Hoshi, Minako M. Journal of Biological Chemistry, Vol. 284, Issue 8 https://doi.org/10.1074/jbc.R800036200	journal	October 2008
Pt–Ni(OH)2 nanosheets amplified two-way lateral flow immunoassays with smartphone readout for quantification of pesticides Cheng, Nan; Shi, Qiurong; Zhu, Chengzhou Biosensors and Bioelectronics, Vol. 142 https://doi.org/10.1016/j.bios.2019.111498	journal	October 2019
A paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers Sanjay, Sharma T.; Dou, Maowei; Sun, Jianjun Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep30474	journal	July 2016
FeOx-supported platinum single-atom and pseudo-single-atom catalysts for chemoselective hydrogenation of functionalized nitroarenes Wei, Haisheng; Liu, Xiaoyan; Wang, Aiqin Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms6634	journal	December 2014
Single-step immunoassays and microfluidic droplet operation: Towards a versatile approach for detection of amyloid-β peptide-based biomarkers of Alzheimer’s disease Mai, Thanh Duc; Ferraro, Davide; Aboud, Nacéra Sensors and Actuators B: Chemical, Vol. 255 https://doi.org/10.1016/j.snb.2017.09.003	journal	February 2018
Carbon nanotube-linked hollow carbon nanospheres doped with iron and nitrogen as single-atom catalysts for the oxygen reduction reaction in acidic solutions Li, Jin-Cheng; Cheng, Min; Li, Tao Journal of Materials Chemistry A, Vol. 7, Issue 24 https://doi.org/10.1039/C9TA00508K	journal	January 2019
N-doped carbon nanotubes containing a high concentration of single iron atoms for efficient oxygen reduction Li, Jin-Cheng; Yang, Zhi-Qing; Tang, Dai-Ming NPG Asia Materials, Vol. 10, Issue 1 https://doi.org/10.1038/am.2017.212	journal	January 2018
Single-Atom Catalysts: Emerging Multifunctional Materials in Heterogeneous Catalysis Zhang, Huabin; Liu, Guigao; Shi, Li Advanced Energy Materials, Vol. 8, Issue 1 https://doi.org/10.1002/aenm.201701343	journal	September 2017
Review—Nanozyme-Based Immunosensors and Immunoassays: Recent Developments and Future Trends Niu, Xiangheng; Cheng, Nan; Ruan, Xiaofan Journal of The Electrochemical Society, Vol. 167, Issue 3 https://doi.org/10.1149/2.0082003JES	journal	November 2019
Improved assessment of accuracy and performance indicators in paper-based ELISA Mazzu-Nascimento, Thiago; Morbioli, Giorgio Gianini; Milan, Luis Aparecido Analytical Methods, Vol. 9, Issue 18 https://doi.org/10.1039/C7AY00505A	journal	January 2017
Single-atom nanozymes Huang, Liang; Chen, Jinxing; Gan, Linfeng Science Advances, Vol. 5, Issue 5 https://doi.org/10.1126/sciadv.aav5490	journal	May 2019
Hierarchically Porous M-N-C (M = Co and Fe) Single-Atom Electrocatalysts with Robust MN _x Active Moieties Enable Enhanced ORR Performance Zhu, Chengzhou; Shi, Qiurong; Xu, Bo Z. Advanced Energy Materials, Vol. 8, Issue 29 https://doi.org/10.1002/aenm.201801956	journal	August 2018
Amyloid-β protein oligomerization and the importance of tetramers and dodecamers in the aetiology of Alzheimer's disease Bernstein, Summer L.; Dupuis, Nicholas F.; Lazo, Noel D. Nature Chemistry, Vol. 1, Issue 4 https://doi.org/10.1038/nchem.247	journal	June 2009

Similar Records

Unprecedented peroxidase-mimicking activity of single-atom nanozyme with atomically dispersed Fe–Nx moieties hosted by MOF derived porous carbon

Journal Article · Tue Oct 01 00:00:00 EDT 2019 · Biosensors and Bioelectronics · OSTI ID:1691526

Niu, Xiangheng; Shi, Qiurong; Zhu, Wenlei; +8 more

Gold core/Ceria shell-based redox active nanozyme mimicking the biological multienzyme complex phenomenon

Journal Article · Sat Dec 02 00:00:00 EST 2017 · Journal of Colloid and Interface Science · OSTI ID:1691526

Bhagat, Stuti; Srikanth Vallabani, NV; Shutthanandan, Vaithiyalingam; +3 more

Bioelectrochemical Immunoassay of Polychlorinated Biphenyl

Journal Article · Tue Apr 01 00:00:00 EDT 2008 · Analytica Chimica Acta, 612:23-28 · OSTI ID:1691526

Lin, Ying-Ying; Liu, Guodong; Wai, Chien M; +1 more

Related Subjects

60 APPLIED LIFE SCIENCES

Title: Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of A β 1-40: A Biomarker of Alzheimer’s Disease

Citation Formats

References (48)

Similar Records

Related Subjects