Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of A β 1-40: A Biomarker of Alzheimer’s Disease

Lyu, Zhaoyuan; Ding, Shichao; Zhang, Nan; Zhou, Yang; Cheng, Nan; Wang, Maoyu; Xu, Mingjie; Feng, Zhenxing; Niu, Xiangheng; Cheng, Yuan; Zhang, Chao; Du, Dan; Lin, Yuehe

doi:10.34133/2020/4724505

Single-Atom Nanozymes Linked Immunosorbent Assay for Sensitive Detection of A β 1-40: A Biomarker of Alzheimer’s Disease

Journal Article · Mon Oct 19 00:00:00 EDT 2020 · Research

DOI:https://doi.org/10.34133/2020/4724505· OSTI ID:1691526

Lyu, Zhaoyuan ^[1]; ^[1]; Zhang, Nan ^[2]; Zhou, Yang ^[1]; Cheng, Nan ^[1]; Wang, Maoyu ^[3]; Xu, Mingjie ^[4]; ^[3]; Niu, Xiangheng ^[1]; ^[2]; Zhang, Chao ^[5]; ^[1]; ^[1]

Washington State Univ., Pullman, WA (United States)
Inst. of High Performance Computing (Singapore)
Oregon State Univ., Corvallis, OR (United States)
Univ. of California, Irvine, CA (United States)
National Univ. of Singapore (Singapore)

Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-N_x SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Results indicate that the Fe-N_x SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP, owing to the maximized utilization of Fe atoms and their abundant active sites, which could mimic natural metalloproteases structures. Further designed SAN-linked immunosorbent assay (SAN-LISA) demonstrates the ultralow limit of detection (LOD) of 0.88 pg/mL, much more sensitive than that of commercial ELISA (9.98 pg/mL). The results confirm that the Fe-N_x SANs can serve as a satisfactory replacement of enzyme labels, which show great potential as an ultrasensitive colorimetric immunoassay.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1691526

Journal Information:: Research, Journal Name: Research Journal Issue: 10 Vol. 2020; ISSN 2639-5274

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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