Isolation and genetic analysis of extragenic suppressors of the hyper-deletion phenotype of the Saccharomyces cerevisiae hpr1 {Delta} mutation

Journal Article · Sun Jan 01 04:00:00 EST 1995 · Genetics

OSTI ID:255329

Santos-Rosa, H; Aguilera, A ^[1]

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1. Universidad de Sevilla (Spain)

The HPR1 gene of Saccharomyces cerevisae is involved in maintaining low levels of deletions between DNA repeats. To understand how deletions initiate in the absence of the Hpr1 protein and the mechanisms of recombination leading to deletions in S. cerevisiae, we have isolated mutations as suppressors of the hyper-deletion phenotype of the hpr1{Delta} mutation. The mutations defined five different genes called HRS for hyper-recombination suppression. They suppress the hyper-deletion phenotype of hpr1{Delta} strains for three direct repeat systems tested. The mutations eliminated the hyper-deletion phenotype of hpr1{Delta} strains either completely (hrs1-1 and hrs2-1) or significantly (hrs3-1, hrs4-1 and hrs5-1). None of the mutations has a clear effect on the levels of spontaneous and double-strand break-induced deletions. Among other characteristics we have found are the following: (1) one mutation, hrs1-1, reduces the frequency of deletions in rad52-1 strains 20-fold, suggesting that the HRS1 gene is involved in the formation of RAD52-independent deletions; (2) the hrs2-1 hpr1{Delta} mutant is sensitive to methyl-methane-sulfonate and the single mutants hpr1{Delta} and hrs2-1 are resistant, which suggests that the HPR1 and HRS2 proteins may have redundant DNA repair functions; (3) the hrs4-1 mutation confers a hyper-mutator phenotype and (4) the phenotype of lack of activation of gene expression observed in hpr1{Delta} strains is only partially suppressed by the hrs2-1 mutation, which suggests that the possible functions of the Hpr1 protein in gene expression and recombination repair can be separated. We discuss the possible relationship between the HPR1 and the HRS genes and their involvement in initiation of the events responsible for deletion formation. 36 refs., 3 figs., 7 tabs.

• OSTI ID: 255329
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 1 Vol. 139; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA
DNA REPAIR
GENE MUTATIONS
GENE RECOMBINATION
GENE REGULATION
GENES
MUTATION FREQUENCY
PHENOTYPE
PROTEINS
SACCHAROMYCES CEREVISIAE