The yeast HRS1 gene encodes a polyglutamine-rich nuclear protein required for spontaneous and hpr1-induced deletions between direct repeats
Journal Article
·
· Genetics
OSTI ID:539419
- Universidad de Sevilla (Spain)
- Univ. of Bern (Switzerland); and others
The hrs1-1 mutation was isolated as an extragenic suppressor of the hyperrecombination phenotype of hpr1{Delta} cells. We have cloned, sequenced and deleted from the genome the HRS1 gene. The DNA sequence of the HRS1 gene reveals that it is identical to PGD1, a gene with no reported function, and that the Hrs1p protein contains polyglutamine stretches typically found in transcription factors. We have purified a His(6) tagged version of Hrs1p protein from E. coli and have obtained specific anti-Hrs1p polyclonal antibodies. We show that Hrs1p is a 49-kD nuclear protein, as determined bv indirect immunofluorescence microscopy and Western blot analysis. The hrs1{Delta} null mutation reduces the frequency of deletions in wild-type and hpr1{Delta} backgrounds sevenfold below wild-type and rad52 levels. Furthermore, hrs1{Delta} cells show reduced induction of the GAL1,10 promoter relative to wild-type cells. Our results suggest that Hrs1p is required for the formation of deletions between direct repeats and that it may function in gene expression. This suggests a connection between gene expression and direct repeat recombination. In this context, we discuss the possible roles of Hrs1p and Hpr1p in initiation of direct-repeat recombination. 62 refs., 5 figs., 5 tabs.
- OSTI ID:
- 539419
- Journal Information:
- Genetics, Journal Name: Genetics Journal Issue: 3 Vol. 142; ISSN GENTAE; ISSN 0016-6731
- Country of Publication:
- United States
- Language:
- English
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