Direct mapping of seven genes encoding human type 2 cystatins to a single site located at 20p11.2
- Univ. of Texas-Houston Health Science Center, TX (United States); and others
The human type 2 cystatins are encoded by a multigene family of eight to nine members. Five functional genes (CST1-CST5) and two pseudogenes (CSTP1 and CSTP2) have previously been cloned. The authors have developed a panel of short genomic fragments that serve as gene-specific probes for these seven genes under hybridization conditions used for Southern blots or fluorescence in situ hybridization (FISH). Analysis of BssHII-digested genomic DNA using these probes was consistent with association of all seven genes with a 905-kb fragment, suggesting physical clustering at a single site. The cluster was assigned to chromosome 20 by screening a somatic cell hybrid panel with an STS for CST2. This assignment was confirmed for each gene by FISH using repeat-free gene-specific genomic probes ranging in size from 2.3 to 0.8 kb. All the loci mapped to 20p11.2. 17 refs., 2 figs.
- OSTI ID:
- 255226
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 24; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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