Localization of the glycine receptor {alpha}{sub 1} subunit gene (GLRA1) to chromosome 5q32 by FISH
- Women`s and Children`s Hospital, Adelaide (Australia)
- Garvan Institute of Medical Research, New South Wales (Australia)
The human Gly R {alpha}{sub 1} subunit cDNA was labeled by nick-translatoin using biotin-14-dATP and hybridized in situ at a final concentration of 20 ng/{mu}l to metaphase chromosome spreads from two normal males. The FISH method was modifed from that previously described in that chromosomes were stained with both propidium iodide (as a counterstain) and DAPI (for chromosome identification) before analysis. Images of metaphase preparations were caputed by a CCD camera and were computer enhanced.
- OSTI ID:
- 250007
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 22; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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